腺病毒介导miR-122联合化疗药物ADM对肝癌细胞杀伤作用的研究  

Enhanced Inhibitory Effect of Adenovirus-mediated miR-122 Combined with ADM of Growth on Different Hepatocarcinoma Cell Lines

作　　者：徐艳敏[1] 马清华[1] 武佳[1] 李伟[1] 钱程[1] 刘立[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2010年第6期919-923,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：973计划(2004CB518804);浙江省"生物医学工程"重中之重学科开放基金(WYX0816)

摘　　要：采用CCK-8比色分析等方法开展腺病毒介导mi R-122联合化疗药物阿霉素(ADM)针对不同人肝癌细胞的杀伤情况的研究。希望通过对比研究,寻找解决化疗药物在临床使用中所出现的疗效低且毒副作用大等难题的方法。研究显示:低浓度的ADM对肝癌细胞的细胞毒作用比较小,但疗效也较差;当ADM浓度增高至80 ng/mL以上,尤其是80 ng/mL的ADM联合20 MOI Ad-mi R122杀伤各种肝肿瘤细胞的能力明显优于单独使用ADM或Ad-mi R122。To investigate the cytotoxicity effect of adenovirus-mediated miR-122 combined with adriamycin（ADM）on growth of different human hepatocellular carcinoma cell lines by CCK-8 kit.Comparative research can hope to find a solution to the clinical use of chemotherapeutic drugs with low efficacy and high toxicity.The results show that low concentrations of ADM on cytotoxicity of liver cancer cells is relatively small,but the effects are less.When the ADM concentration increases to 80 ng/mL or more,especially 80 ng/mL for ADM combined 20 MOI Ad-miR122 destruction of various liver tumor cells are markedly better than ADM or Ad-miR122 alone.Conclusion： 20 MOI Ad-miR122 combined with 80 ng/mL ADM has enhanced inhibitory effect on growth different human hepatocellular carcionoma cell lines.

关 键 词：ADM Ad-miR122 肝癌细胞株 

分 类 号：Q786[生物学—分子生物学]