山茱萸提取部位对D-半乳糖致衰模型小鼠骨髓细胞脱氧核糖核酸的影响  

Effect of Extracts from Fructus Corni on DNA in Marrow Cells of D-galactose-induced Subacute Aging Mice

作　　者：王明艳[1] 江励华[1] 董菊[1] 许冬青[1] 杜逸芳[2] 俞晓忆[3] 杜伟锋[4] 蔡宝昌[1] 

机构地区：[1]南京中医药大学,江苏省中药炮制重点实验室,江苏南京210029 [2]南京师范大学,江苏南京210005 [3]中国药科大学,江苏南京210009 [4]浙江中医药大学,浙江杭州311401

出　　处：《时珍国医国药》2010年第10期2425-2427,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30772851);国家"十五"科技攻关计划(No.2001BA701A11)

摘　　要：目的探讨山茱萸几种提取部位对D-半乳糖致衰模型小鼠骨髓细胞DNA的影响。方法取ICR小鼠,复制D-半乳糖致衰模型,给小鼠灌服药液,通过单细胞电泳和流式细胞仪检测,观察D-半乳糖致衰模型小鼠骨髓细胞DNA的变化及山茱萸几种提取部位的保护作用。结果 D-半乳糖对小鼠骨髓细胞脱氧核糖核酸(DNA)结构表现出一定的的影响,山茱萸几种提取部位尤其是石油醚萃取部位表现出保护作用。结论山茱萸提取部位对D-半乳糖致衰模型小鼠DNA结构有不同程度的影响。Objective To investigate the effect of extracts from Fructus Corni on DNA of marrow cells in D-galactose induced subacute aging mice. Methods The subacute aging model was established by D-galactose induced in ICR mice,and the DNA change was tested by single-cell electrophoresis and flow cytometry detection. Results D-galactose had the effect on DNA of marrow cells of ICR mice and extracts from Fructus Corni,especially the extract of petroleum showed certain protective effects against DNA damage. Conclusion The extracts from Fructus Corni have effect on DNA of marrow cells in D-galactose-induced subacute aging mice.

关 键 词：山茱萸 提取部位 D-半乳糖 脱氧核糖核酸 

分 类 号：R285.5[医药卫生—中药学]