正交实验法优选沙枣花总黄酮提取工艺及沙枣花醇提物体外抗氧化活性的研究  

Optimization Extraction Technology of Total Flavonoids in Elaeagnus an gustifolia Blossms by Orthogonal Test and Antioxidant Activity of the Alcohol Extrcact in Vitro

作　　者：王基云[1] 陆钊罡[1] 王丹 肖旭[1] 张振华[1] 姚遥[1,3] 王锐[1] 王妍[1] 

机构地区：[1]宁夏医科大学基础医学院,宁夏银川750004 [2]宁夏回族自治区第三人民医院,宁夏银川750000 [3]宁夏医科大学医学科学技术研究中心,宁夏银川750004

出　　处：《时珍国医国药》2010年第10期2487-2489,共3页Lishizhen Medicine and Materia Medica Research

基　　金：教育部春晖计划项目(No.Z2008-1-75014);宁夏回族自治区自然科学基金(No.NZ0892)

摘　　要：目的研究提取沙枣花(elaeagnus angustifolia blossoms,EAB)中总黄酮的最佳工艺条件,并观察了沙枣花醇提物(the alcohol extracts of elaeagnus angustifolia blossoms,AE-EAB)清除氧自由基的作用。方法以芦丁为对照品,NaNO2-Al(NO3)3-NaOH显色体系作为显色剂,采用正交实验方法对沙枣花总黄酮提取方法进行了系统研究。结果最佳提取工艺为20倍量70%乙醇回流3次,1h/次。AE-EAB对氧自由基的清除作用随着浓度的升高而增强。结论 EAB总黄酮的最佳提取工艺为A2B2C1D3。AE-EAB对氧自由基有清除作用.Objective To study the optimal extraction technology of total flavonoids in Elaeagnus angustifolia blossoms（ EAB） , and the free radicals scavenging effect of the alcohol extract of Elaeagnus angustifolia blossoms（ AE-EAB ） . Methods Using Rutin as the evaluating criteria and NaNO2 -Al（ NO3） 3-NaOH as chromogenic agent,the extraction condition was studied by orthogonal test. Results The optimal technological conditions were attained by the orthogonal experiment as follows： extraction with70% ethanol,ethanol volume 20 ml for 1 g EAB every time,and extracting for 3 times （ 40 min for each time） . The scavenging effect on oxygen free radical was proportional to the concentration of AE-EAB. Conclusion The optimum extraction technology is A2B2C1D3 . AE-EAB has scavenging effect on oxygen free radical .

关 键 词：沙枣花 总黄酮 紫外分光光度法 正交实验 氧自由基 

分 类 号：R285.5[医药卫生—中药学]