桃核承气汤对庆大霉素肾毒性大鼠的治疗作用研究  

Therapeutic Effect of Taohe Chengqi Decoction on Rat Kidney Lesion Induced by Gentamicin

作　　者：刘煜敏[1] 张悦[1] 郝艳鹏[1] 王飞[1] 陆敏[1] 

机构地区：[1]上海中医药大学科技实验中心,上海201203

出　　处：《时珍国医国药》2010年第10期2607-2609,共3页Lishizhen Medicine and Materia Medica Research

基　　金：上海高校选拔培养优秀青年教师科研专项基金(No.SZY08044);上海市教委重点学科(第五期)J50301;上海高校创新团队建设项目

摘　　要：目的观察桃核承气汤对庆大霉素所致大鼠肾毒性损伤模型的治疗作用及部分机制。方法采用腹腔注射庆大霉素法制作大鼠肾毒性模型,通过生化、病理检测和蛋白免疫印迹方法观察各组大鼠肾功能、肾组织病理形态、氧化产物及酶类的变化及信号通路分子p38、核因子κB(NF-κB)蛋白表达的变化,明确桃核承气汤的疗效。结果桃核承气汤治疗一周后,可改善肾功能,减轻肾组织病理改变,显著降低肾皮质MDA含量和NOS酶活性、提高SOD酶活性,NF-κB蛋白表达明显减少。结论桃核承气汤可以通过改善肾脏氧化损伤对庆大霉素肾毒性起到一定的治疗作用。Objective To study the therapeutic effect of Taohe Chengqi Decoction （ TCD） on gentamicin-induced kidney lesion in rats. Methods The renal injury model was established by Gentamicin i. p. The kidney index,pathomorphology,and level of oxidative production and expression of Nuclear Factor-κB （ NF-κB） protein in kidney cortex were measured to observe the effect of TCD on kidney injury of rats. Results After one week therapy,parameters of kidney indexes,oxidative production MDA and enzyme activity of NOS in kidney cortex of Gentamicin group were significantly decreased （ P 0. 05） ,and enzyme activity of SOD was significantly increased（ P 0. 05） . The protein expression of NF-κB was significantly down-regulated （ P 0. 05） . Conclusion Taohe Chengqi Decoction has some therapeutic effect on Gentamicin-induced kidney lesion through alleviating renal oxidation.

关 键 词：桃核承气汤 庆大霉素 核因子ΚB 肾毒性 

分 类 号：R285[医药卫生—中药学]