低能N^+束诱发对狗牙根种子发芽能力的影响  

Effect of low energy N^+ beams on germination ability of Cynodon dactylon seeds

作　　者：王利宝[1] 刘瑞峰[1] 刘卫东[1] 

机构地区：[1]中南林业科技大学林学院,湖南长沙410004

出　　处：《草业科学》2010年第10期43-47,共5页Pratacultural Science

基　　金：中南林业科技大学青年基金项目(101-0719);湖南省教育厅重点项目(08A028)

摘　　要：探索低能N+束对狗牙根(Cynodon dactylon)的诱变效应及其机理,研究M1代种子发芽状况为确定其适宜的诱变参数奠定基础。通过LZD-1000离子注入机将N+束注入狗牙根干种子,研究N+束引起种子发芽能力的变化。结果表明,注入一定剂量的N+束可以促进狗牙根种子发芽,但注入时间以5 s为宜,超过5 s其发芽能力急剧下降。5 s注入时间下6种剂量处理的种子发芽率、发芽势和活力指数均值分别高出对照10%、9%和16,10 s注入时间下3项指标均值分别低于对照55%、15%和134,不同剂量对种子发芽率、发芽势及活力指数的影响存在显著差异(3项指标F值分别为638.13、109.31和2.16,均大于F0.05(12,26)=2.15)。在20 keV能量下,注入时间为5 s和剂量为80×1014ions/cm2是N+束注入诱变狗牙根育种最有效的诱变参数,可诱使狗牙根种子萌发状况发生较大的变异。Study on germination of M1 generation seeds from Cynodon dactylon was to establish feasible implantation parameters and to understand mutation effect of N^＋ beams on C.dactylon and its mechanism.Naturally dry seeds were treated through N^＋ beams from LZD-1000 machine,and the change on germination ability of seeds aroused from N^＋ beams was studied.The results indicated that the appropriate dose of N^＋ beams was able to stimulate seeds germinating if the implantation time was in 5 seconds,the germination ability would decrease if over 5 seconds.Three values of germination rate,germination energy and vigor index of seeds through six dose treatments with 5 seconds were more than that in control by 10%,9% and 16.But three values of controlled seeds with 10 s treatment time were lower than control by 55%,15% and 134.There were significantly differences in effect of different doses on germination rate,germination energy and vigor index（the F values of three indexes were 638.13,109.31,2.16,and higher than F0.05（12,26）=2.15）.It is concluded that 5s implantation time and 80×10^14 ions/cm^2 dosage were best mutation parameters under 20 kev energy,and this could induce more variation on seed germination of C.dynodon.

关 键 词：狗牙根 低能N^+束 诱变参数 发芽能力 

分 类 号：S688.4[农业科学—观赏园艺]