针刺对发育期脑损伤大鼠脑组织细胞凋亡及Bcl-2、Bax蛋白表达的影响  

Acupuncture Effect on apoptosis and Bcl-2,Bax protein expression in brain damage rats of intrauterine ischemia

作　　者：吴至凤[1] 温恩懿[1] 赵聪敏[1] 赵雪晴[1] 赵永娇[1] 

机构地区：[1]第三军医大学新桥医院儿科,重庆400037

出　　处：《重庆医学》2010年第21期2898-2900,2903,共4页Chongqing medicine

摘　　要：目的观察针刺疗法对宫内缺血脑损伤大鼠脑组织细胞凋亡及其凋亡相关蛋白Bcl-2、Bax表达的影响,为针刺疗法应用于发育期脑损伤提供实验依据。方法采用结扎孕鼠双侧子宫动脉,完全阻断血供25 min娩出胎鼠,制成宫内窘迫脑损伤模型,将成功的模型随机分为针刺组和模型组,每组各8只;正常组剖宫产取出胎鼠,8只;其余胎鼠丢弃。正常组和模型组不治疗,针刺组于出生后7~30 d行针刺治疗,每日针刺1次,不留针。30 d实验结束后断头取脑,运用TUNEL法检测脑细胞凋亡,SABC法测脑组织Bcl-2、Bax蛋白表达。结果正常组大鼠脑细胞凋亡数极少,而模型组较高,针刺组介于两者之间,3组差异有统计学意义（P〈0.05）;Bcl-2染色指数正常组或针刺组比模型增加,差异有统计学意义（P〈0.05）;Bax染色指数正常组或针刺组比模型组降低,差异有统计学意义（P〈0.05）。结论针刺可以减少宫内窘迫脑损伤大鼠脑组织神经细胞凋亡,提高Bcl-2蛋白的表达,降低Bax蛋白的表达,这可能是应用针刺疗法治疗发育期脑损伤的机制之一。Objective To observe the acupuncture effect on apoptosis and Bcl-2,Bax protein expression in intrauterine ischemic brain damage rats′ brain cells,so as to provide experimental evidence in application of acupuncture to treat developmental brain damage.Methods To ligate bilateral uterine arteries of the pregnant rats,for completely blocking the blood supply for 25 min,then deliver fetus rats to make the intrauterine ischemic brain damage models.The successful-models were randomly divided into the acupuncture group and the model group,8 rats each.8 rats in the normal group were delivered by caesarean section.The remaining fetal rats were discarded.Normal group and model group were not treated,the acupuncture group′s rats were given acupuncture treatment from 7-30 d,once a day,without remaining needles.On 30 d which was the end of the experiment,all the rats′ brains were removed.Using TUNEL assay apoptosis of brain cells,SABC method to measure brain Bcl-2,Bax protein expression.Results The index of Bcl-2 staining in the model group or acupuncture group comparing with the normal group was significantly different（P〈0.05）,while there was a significant difference between the acupuncture group and the model group（P〈0.01）.Comparing brain cells′ apoptosis of the 3 groups,the normal group was the fewest,while the model group was the highest,the acupuncture group was between the two groups,the three groups had significant difference（P〈0.05）.Conclusion Acupuncture can reduce the apoptosis of the brain neuronal cells in intrauterine ischemic brain damage rats,and can also improve the Bcl-2 protein expression and reduce the Bax protein expression of the brain neuronal cells,which may be one of the mechanisms how acupuncture therapy treating the developmental brain damage.

关 键 词：缺氧缺血 脑损伤 细胞凋亡 Bcl-2 BAX 针刺疗法 

分 类 号：R245[医药卫生—针灸推拿学] R446.61[医药卫生—中医临床基础]