姜黄素在体外对氨基酸递质转运体EAAC1及GAT1功能的影响  

Effects in vitro of Curcumin on the Electrophysiological Property of EAAC1 and GAT1

作　　者：李鑫[1] 杜鹏[1] 汪昕[1] 

机构地区：[1]复旦大学附属中山医院,上海200032

出　　处：《中国临床医学》2010年第5期638-641,共4页Chinese Journal of Clinical Medicine

基　　金：上海市卫生局青年科学基金(2008);复旦大学脑科学研究院开放课题基金(2008)

摘　　要：目的:探讨体外条件下姜黄素对兴奋性氨基酸递质转运体EAAC1及抑制性递质转运体GAT1功能活性的影响。方法:利用爪蟾卵母细胞内源性Na/K-ATPase及外源表达的大鼠Na/K-ATPase、EAAC1及GAT1蛋白,借助双电极电压钳技术,应用姜黄素干预,实时记录并观察姜黄素干预对不同离子转运体介导稳态电流的影响。结果:(1)不同浓度梯度姜黄素(7.5μmol.L-1、15μmol.L-1、30μmol.L-1、75μmol.L-1)灌流对内源性及外源性Na/K-ATPase介导稳态电流,无显著影响;不同浓度姜黄素(15μmol.L-1和30μmol.L-1,)预处理卵母细胞24h,亦未观察到明显效应;借助微量注射方法,将姜黄素(30μmol.L-1)直接注射到卵母细胞内,对Na/K-ATPase介导稳态电流无显著影响。(2)30μmol.L-1姜黄素灌流对外源性EAAC1介导稳态电流无明显影响。(3)30μmol.L-1姜黄素灌流可以轻度抑制外源性GAT1介导稳态电流。结论:姜黄素对细胞膜Na/K-ATPase和EAAC1介导稳态电流无显著影响,对GAT1介导稳态电流有轻度抑制作用。Objective：To elucidate the possible effect of curcumin on the electrophysiological property of Na/K-ATPase,EAAC1 and GAT1.Methods： The Xenopus Laevis oocytes were utilized as a protein expression model and heterogeneous cRNA of SD rats were expressed in the oocytes.Two-electrode voltage clamp was employed to detect the steady-state current induced by these proteins.Curcumin was administered to the oocytes via different methods respectively,perfusion,incubation or intracellular injection,for example.The changes before and after the treatment of curcumin were observed.Results：（1） Application of a series list of curcumin（7.5 μmol/L,15 μmol/L,30 μmol/L and 75 μmol/L） could not affect the stead-state current induced by Na/K pump.Neither could curcumin by the protocol of incubation or intracellular injection.（2） Current of EAAC1 was not sensitive to curcumin（30 μmol/L） either.（3） Current of GAT1 was slightly decreased after the treatment of curcumin 30 μmol·L^-1.Conclusions： Curcumin could not affect the current induced by Na/K-ATPase or EAAC1,but could slightly suppress the current induce by GAT1.

关 键 词：姜黄素 钠钾泵 兴奋性氨基酸转运体 γ氨基丁酸转运体 

分 类 号：R285.5[医药卫生—中药学]