滋养层细胞的分离培养与鉴定  

Isolation and Differentiation of Human First Trimester Trophoblasts

作　　者：王海霞[1] 李金枝[1] 解其贵[1] 杨瑜[2] 董凌云[1] 左绪磊[1] 

机构地区：[1]复旦大学附属金山医院妇产科,上海200540 [2]复旦大学附属公共卫生中心科研部,上海201508

出　　处：《中国临床医学》2010年第5期724-726,共3页Chinese Journal of Clinical Medicine

摘　　要：目的：培养符合实验要求的人绒毛滋养层细胞。方法：胰蛋白酶、胶原酶消化绒毛组织,进行原代培养,用胰蛋白酶消化法进行传代培养并纯化。观察其形态学特征并绘制生长曲线,计算倍增时间。应用光镜、免疫荧光进行细胞鉴定。Tran-swell小室法检测其体外侵袭能力。结果：原代培养滋养层细胞24h贴壁,7~10d首次传代,倍增时间3.42d,细胞表达细胞角蛋白7而不表达波形蛋白。结论：胰蛋白酶、胶原酶消化法可获得符合实验要求的人绒毛滋养层细胞,能建立稳定的人绒毛滋养层细胞体外培养体系。Objective：To culture human first trimester cytotrophoblasts in vitro.Methods： Human first trimester cytotrophoblasts were isolate,purify and culture.The morphology characteristics were detected through microscopy,we drew growth curve and calculated the doubling time to describe its growth characteristics.Immunofluorescence was carried out to determined the expression of cytokine 7 and vimintin.Results： The cells adherence achieved 24-48 hours after seeding,7-10 days later the cells were subcultured for the first time.The doubling time of cell population was 3.4 days.The cell populations expressed cytokeratin 7 but not vimentin.Transwell invasion experiment showed that the invasive capability remained in vitro.Conclusions： Typsin-collagenase digestion process can obtain human trophoblastic cells and also can establish a stable human trophoblastic cells culture systerm in vitro.

关 键 词：滋养层细胞 体外培养 

分 类 号：Q813.11[生物学—生物工程]