TGF-β_1诱导肺泡上皮细胞间质转化  

TGF-β_1 induced epithelial-mesenchymal transition of alveolar epithelial cells

作　　者：黄振杰[1] 郑金旭[2] 莫凯天[1] 苏石芳[1] 许世锋[1] 汤艳[2] 石益海[3] 

机构地区：[1]广西北海市人民医院呼吸科,广西北海536000 [2]江苏大学附属医院呼吸科,江苏镇江212001 [3]上海市第八人民医院消化科,上海200235

出　　处：《吉林医学》2010年第29期5062-5064,共3页Jilin Medical Journal

基　　金：卫生部科研基金(编号:wkj2006-2-026);江苏省"333工程"基金(苏人才办2007-16-09)

摘　　要：目的:通过观察TGF-β1诱导下A549细胞出现的细胞形态学和E-cad表达的变化,探讨上皮细胞-间质细胞转化(epithelial-mesenchymal transition,EMT)过程在肺纤维化发病机制中的作用。方法:体外培养A549细胞,以TGF-β1进行干预,收集不同时段的细胞,应用荧光实时定量PCR(RT-PCR)检测TGF-β1干预前后E-cad的mRNA表达变化;倒置相差显微镜观察细胞形态学的变化;间接免疫荧光观察E-cad蛋白表达的变化。结果:倒置相差显微镜观察到TGF-β1干预后A549细胞由鹅卵石状变为梭形,形态如同肌纤维母细胞。间接免疫荧光显示A549细胞的E-cad表达(红色荧光染色)随时间延长逐渐减少。RT-PCR显示E-cad的mRNA表达下调(P<0.05)。结论:TGF-β1在体外诱导肺泡上皮细胞向间质细胞转化,肺泡上皮细胞间质转化是肺纤维化的重要发病机制之一。Objective By observing the cellular morphology changes and the expression of E-cad after A549 cells were treated with transforming growth factorβ1 （ TGF-β1 ） ,to investigate the role of epithelial-mesenchymal transition （ EMT） in the pathogenesy of pulmonary fibrosis.Method A549 cells cultured in vitro were treated by TGF-β1 ,then harvested at different time points to assay mRNA expression of E-cad by real-time PCR （ RT-PCR） before and after A549 cells being treated by TGF-β1.Cellular morphology changes were observed by phase-contrast microscope.Protein expression of E-cad by indirect immunofluorescence.Results After being treated by TGF-β1 ,A549 cells were observed by inverted phase contrast microscope to turn from pebble shape to fusiform shape,a myofibroblast-like morphology.Indirect immunofluorescence showed protein expression of E-cad reduced as time went by（ red stain） .The mRNA expression of E-cad was down-regulated in RT-PCR（ P 0.05） .Conclusion TGF-β1 induced EMT of alveolar epithelial cells in vitro suggests that EMT of alveolar epithelial cells might be one of fundamental mechanisms of pulmonary fibrosis.

关 键 词：转化生长因子Β1 A549细胞 上皮细胞间质转化 肺纤维化 

分 类 号：R563[医药卫生—呼吸系统]