扬子鳄消化道内分泌细胞的免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDIES ON ENDOCRINE CELLS IN THE DIGESTIVE TRACT OF ALLIGATOR SINENSIS

作　　者：吴孝兵[1] 张盛周[1] 陈壁辉[1] 邓泽沛[2] 周占祥[2] 王朝林[3] 聂继山 谢万树 

机构地区：[1]安徽师范大学生物学系,芜湖241000 [2]中国农业大学动物医学院,北京100094 [3]安徽省扬子鳄繁殖研究中心,宣州242000

出　　处：《动物学报》1999年第2期155-161,共7页ACTA ZOOLOGICA SINICA

基　　金：安徽省自然科学基金!95-生-01

摘　　要：应用7种特异性胃肠激素抗血清对扬子鳄（Alligatorsinensis）消化道内分泌细胞进行了免疫组织化学定位。5-羟色胺细胞在消化道各段都有分布，以十二指肠密度最高，食道、直畅其次。生长抑素细胞在胃幽门部非常密集，胃体中等，胃贲门部较少，十二指肠偶见。胃泌素细胞主要分布于十二指肠前段，空肠、回肠和直肠偶见。许多血管活性肠肽细胞分布于胃贲门部，胃体和胃幽门部少数。胰高血糖素、胰多肽和P-物质在消化道各段均未检出阳性细胞。结合扬子鳄的食性，对内分泌细胞的分布型进行了讨论。The distribution and density of the endocrine cells in the digestive tract of Alligator sinensis were studied by immunohistochemical method with seven antisera of gut hormone. 5-Hydroxytryptamine (5-HT)-immunoreactive cells are distributed throughout the digestive tract with the highest density in the duodenum, the second highest in the oesophagus and rectum. Somatostatin-immunoreactive cells occured very numerously in the stomachus Pyloricus, moderately in the stomachus corpus, rarely in the stomachus cardiacus and occasionally in the duodenum. The gastrin-immunoreactive cells were located numerously in the duodenum and occasionally in the jejunum, ileum and rectum.The vasoactive intestinal polypeptide(VIP)-immunoreaetive cells were present mainly in the stomachus cardiacus, rarely in the stomachus corpus and pyloricus. Cells showing immunoreactivity to pancreatic polypeptide, glucagon and substance-P were not detected. Considering the feeding habit, the distributive mode of endocrine cells in the digestive tract of Alligator sinensis was discussed.

关 键 词：扬子鳄 消化道 内分泌细胞 免疫组织化学 

分 类 号：Q959.64[生物学—动物学]