BrUTP标记的小麦核仁中RPⅠ转录位点  

Transcriptive Sites of RNA Polymerase Ⅰ in the Nucleolus of Triticum aestivum Revealed by BrUTP Labelling and Immunoelectron Microscopy 

作　　者：王华春[1] 邢苗[1] 

机构地区：[1]深圳大学

出　　处：《Acta Botanica Sinica》1999年第5期553-555,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家教委资助优秀年轻教师基金

摘　　要：核仁是细胞核中最明显的结构之一，是绝大多数真核细胞中基因转录最为活跃的区域，包括纤维中心（ｆｉｂｒｉｌａｒｃｅｎｔｅｒ，ＦＣ）、致密纤维组分（ｄｅｎｓｅｆｉｂｒｉｌｌａｒｃｏｍｐｏｎｅｎｔ，ＤＦＣ）、颗粒组分（ｇｒａｎｕｌａｒｃｏｍｐｏｎｅｎｔ，Ｇ...The intact meristematic cells of Triticum aestivum L. were labelled with BrUTP and then detected with an anti BrdU antibody and protein A gold, and a large number of gold particles representing newly synthesized RPⅠ transcripts were observed over the nucleolus of the cells. Most of the gold particles were located in the electron dense area, which corresponds to DFC (dense fibrillar component), and the periphery of FC (fibrillar center), while the centre of FC and condensed chromatin showed few gold particles, suggesting that sites of RPⅠ transcription are mainly located in DFC and the periphery of FC. Gold particles in DFC were frequently clustered and lined. The nucleolus associated body (NAB) was often observed in the nucleus. It was a fibrillo granular body closely associated with the nucleolus, and in which were concentrated gold particles representing the newly synthesized RNA.

关 键 词：BrUTP标记 核仁 核仁附着小体 小麦 

分 类 号：S512.101[农业科学—作物学]