Involvement of Calcium dependent Protein Kinases in ABA regulation of Stomatal Movement   

植物细胞钙依赖型蛋白激酶参与ABA信号转导过程的实验证据(英文)

作　　者：王喜庆[1,2] 武维华[1,2] 

机构地区：[1]中国农业大学生物学院植物科学系 [2]西北农业大学基础科学系

出　　处：《Acta Botanica Sinica》1999年第5期556-559,共4页Acta Botanica Sinica（植物学报：英文版）

摘　　要：Patch clamp techniques were employed to investigate if calcium dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA) in Vicia faba. Stomatal opening was completely inhibited by external application of 1 μmol/L ABA, and such ABA inhibition was significantly reversed by the addition of CDPK inhibitor trifluoperazine (TFP). The inward whole cell K + currents were inhibited by 60% in the presence of 1 μmol/L intracellular ABA, and this inhibition was completely abolished by the addition of CDPK competitive substrate histone Ⅲ S. The results suggest that CDPKs may be involved in the signal transduction cascades of ABA regulated stomatal movements.利用膜片钳技术探讨了植物细胞钙依赖型蛋白激酶（ＣＤＰＫ）是否参与了植物激素脱落酸（ＡＢＡ）调控气孔运动的信号转导过程。胞外加入１μｍｏｌ／ＬＡＢＡ完全抑制光照条件下蚕豆叶片气孔的开放，同时加入ＣＤＰＫ抑制剂三氟拉嗪则显著降低ＡＢＡ对气孔开放的抑制作用。在胞内加入１μｍｏｌ／ＬＡＢＡ抑制全细胞内向钾电流约６０％，同时加入ＣＤＰＫ的底物组蛋白ⅢＳ则可完全逆转ＡＢＡ对内向钾电流的抑制作用。研究结果证明，ＣＤＰＫ可能通过对保卫细胞内向钾离子通道的调节介导了ＡＢＡ调控气孔运动的信号转导过程。

关 键 词：Calcium  dependent protein kinases (CDPKs) K  +  channels Abscisic acid Stomatal guard cells 

分 类 号：Q942.5[生物学—植物学]