高效液相色谱法测定厚朴中7种代谢产物含量  

Simultaneous Determination of Seven Metabolites of Magnolia officinalis Rend. et Wils. by High Performance Liquid Chromatography Method

作　　者：方小平[1] 乙引[1] 余正文[1] 杨占南[2] 胡光平[1,2] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001 [2]贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵州贵阳550001

出　　处：《安徽农业科学》2010年第24期13054-13057,共4页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技攻关项目(黔科合GY(2007)3027)

摘　　要：[目的]采用高效液相色谱(HPLC)法同时测定厚朴(Magnolia officinalis Rend.et Wils.)中7种代谢产物含量。[方法]色谱条件:Shim-pack CLC-ODS色谱柱(150mm×6mm,5μm);检测波长:320和294nm;流动相:A相为乙腈,B相为0.1%甲酸,采用梯度洗脱。[结果]峰面积(y)对绿原酸、芦丁、金丝桃苷、槲皮苷、槲皮素、厚朴酚和和厚朴酚对照品溶液浓度(x)的回归方程分别为:y=1E+05x-306360(r=0.9998)、y=5E+04x-79920(r=0.9994)、y=5E+04x-55280(r=0.9999)、y=5E+04x-60530(r=0.9999)、y=2.5E+04x-57010(r=0.9998)、y=2E+04x+7102(r=0.9999)和y=2E+04x+11028(r=0.9999),各对照品溶液在3.125～100.00μg/ml内与峰面积有良好的线性关系。绿原酸、芦丁、金丝桃苷、槲皮苷、槲皮素、厚朴酚和和厚朴酚对照品的加样回收率分别为103.98%、101.65%、99.17%、103.59%、103.59%、102.44%、104.62%,RSD分别为3.37%、2.86%、3.02%、2.89%、2.86%、1.18%、3.67%。厚朴中各部位7种化合物含量存在一定的差异,在叶中,7种代谢产物均有积累,其中和厚朴酚、厚朴酚和槲皮苷的含量较高,金丝桃苷和槲皮素的含量较低。[结论]该研究建立的HPLC法简捷、方便,稳定性和重复性好,可为进一步开发利用厚朴提供依据,并为同时测定黄酮类化合物及酚类化合物提供新的方法。[Objective] The research aimed to simultaneously determine seven metabolite contents of Magnolia officinalis Rend. et Wils. by HPLC method. [Method] The chromatogram conditions were as follows：Shim-pack CLC-ODS chromatographic column,detective wavelength was 320 and 294nm,mobile phase was phase A acetonitrile and phase B 0.1% formic acid,the velocity was seted according to the setted gradient condition. [Result] The concentrations of chlorogenic acid,rutin,hyperin,querctrin,quercetin,magnolol and honokiol liquid （ x ） determined by HPLC method had good linear relationship with the peak areas（ y ） when their concentrations were at 3.125-100.00 μg/ml,and their formulas were y =1E＋05 x-306 360（ r =0.999 8）、 y =5E＋04 x-79 920（ r =0.999 4）、 y =5E＋04 x-55 280（ r =0.999 9）、 y =5E＋04 x-60 530（ r =0.999 9）、 y =2.5E＋04 x-57 010（ r =0.999 8）、 y =2E＋04 x ＋7 102（ r =0.999 9） and y =2E＋04 x ＋11 028（ r =0.999 9） respectively. The average recoveries of chlorogenic acid,rutin,hyperin,querctrin,quercetin,honokiol and magnolol samples were 103.98%,101.65%,99.17%,103.59%,103.59%,102.44% and 104.62%,and their RSD were 3.37%,2.86%,3.02%,2.89%,2.86%,1.18% and 3.67%. The contents of chlorogenic acid,rutin,hyperin,querctrin,quercetin,honokiol and magnolol in Magnolia officinalis Rend. et Wils. had some difference. And the 7 metabolites were all existed in its leaf,thereinto,the contents of honokiol,magnolol and querctrin were high,while hyperin and quercetin were low. [Conclusion] The method established by this study was simple,convenient,feasible and repeatabl,can provide basis for the further development and utilization of Magnolia officinalis Rend. et Wils..

关 键 词：厚朴 代谢产物 HPLC法 

分 类 号：S567.11[农业科学—中草药栽培]