枫叶秋海棠组织培养与快繁技术的研究  

Study on the Rapid Propagation with Tissue Culture of Begonia heracleifoniana

作　　者：宗宪春[1] 宗灿华[2] 

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157012 [2]牡丹江医学院,黑龙江牡丹江157012

出　　处：《安徽农业科学》2010年第24期13561-13562,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]通过组织培养方法筛选枫叶秋海棠不定芽诱导、伸长及生根的最佳培养基,为枫叶秋海棠的快速繁殖提供参考。[方法]以枫叶秋海棠的叶柄、叶片为外植体,进行不定芽诱导、伸长及生根的组织培养。[结果]枫叶秋海棠叶片最佳芽诱导、芽伸长培养基为MS+ZT(2.0mg/L)+NAA(0.2mg/L)。生根的最佳培养基为1/2MS+IBA(0.2mg/L),试管苗移栽后成活率为100%。[结论]该试验建立了枫叶秋海棠组织培养快速繁殖体系,为枫叶秋海棠的快速繁殖和遗传转化提供理论依据。[ Objective ] The study aimed to screen the optimum medium for the induction elongation of adventitions bud, and rooting of Begonia heracleifoniana and provide reference for the rapid propagation of Begonia heracleifoniana. [ Method] With leaves and petiole of Begonia heracleifolia as explants, the culture condition of the induction of adventitions bud and rooting were studied. [ Result] The optium medium for the differentiation and elongation of leaves from Begonia heracleifonia was MS ＋ ZT（2.0 rag/L） ＋ NAA（0.2 mg/L）. The optimum medium for the rooting of it was 1/2MS ＋ IBA（0.2 rag/L）, and the survival rate of test tube seedling was 100% after being transplanted. [ Conclusion ] The rapid propagation system of Begonia heracleifoniana was established in this study,which would provide theoretical evidence for the rapid propagation and genetic transformation of Begonia heracleifoniana.

关 键 词：枫叶秋海棠 组织培养 快速繁殖 

分 类 号：S41[农业科学—植物保护]