羊耳菊提取物的抑菌动力学研究  

Antimicrobial Dynamics of Inula cappa Extracts

作　　者：刘胜贵[1,2] 陈富城[1] 李军[1] 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]怀化学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008

出　　处：《安徽农业科学》2010年第26期14322-14324,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究羊耳菊不同浓度提取物对细菌存活率随时间变化的关系,以及不同pH值条件下提取物的抑菌效果。[方法]以金黄色葡萄球菌、甲型副伤寒沙门氏菌为供试菌种,绘制时间杀菌曲线和浓度杀菌曲线。在提取物浓度为最低抑菌浓度(MIC)时培养24h,测定不同pH值条件下的抑菌率。[结果]羊耳菊提取物对金黄色葡萄球菌和甲型副伤寒沙门氏菌的MIC、最低杀菌浓度(MBC)分别为62.5、125.0mg/ml和125.0、250.0mg/ml。绘制了羊耳菊提取物16MIC、4MIC、2MIC、MIC、1/2MIC和对照条件下的杀菌曲线。羊耳菊水提物抑菌作用最适pH值为6～8。[结论]羊耳菊提取物对金黄色葡萄球菌的杀菌作用在较高浓度时并没有进一步提高杀菌速度,表现为时间依赖性,但对甲型副伤寒沙门氏菌表现为浓度性依赖性。[Objective] In order to investigate the effects of different concentrations of Inula cappa extracts on viability of Staphylococcus aureus and Salmonella paratyphi for various time exposures.[Method] The time-killing curves and concentration-killing curves against S.aureus and S.paratyphi were investigated at the concentration of 1/2×MIC,1×MIC,2×MIC,4×MIC and 16×MIC,and the sterilization rate of Inula cappa on the microorganisms was determined at different pH.[Result] The MICs and MBC of Inula cappa against S.aureus and S.paratyphi are 62.5,125.0 and 125.0,250.0 mg/ml respectively.Optimum pH values of the extracts of Inula cappa against S.paratyphi are 6-8.[Conclusion] Results indicated that the plant extracts could be time-dependent for S.aureus,and a concentration-dependent for S.paratyphi.

关 键 词：羊耳菊提取物 最低抑菌浓度 最低杀菌浓度 抑菌动力学 最适PH值 

分 类 号：S682.11[农业科学—观赏园艺]