转基因在玉米中的遗传分离与整合特性的研究  

Studies of Transgene Segregation and Integration in Maize

作　　者：王守才[1] 王国英[1] 丁群星[1] 张宏[1] 谢友菊[1] 戴景瑞[1] 

机构地区：[1]中国农业大学,北京100094

出　　处：《Acta Genetica Sinica》1999年第3期254-261,共8页

基　　金：国家八六三计划;高等学校博士学科点专项科研基金!930121

摘　　要：用PCR和DNA分子杂交方法研究了转基因Bt在3种不同方法获得的8个转化体后代中的遗传分离、整合性质及其稳定性，结果表明：（1）转基因在大多数转化体后代中呈简单的孟德尔遗传。在R_1至R_2代，部分家系中转化体比例偏低，有的发生转基因丢失，但到R_3代以后均趋于正常的孟德尔遗传方式，在群体中固定下来；（2）转基因在不同转化体中的整合类型有一定差异，但整合的位点和拷贝数都较少，区多呈串联或紧密连锁的整合；（3）在部分家系中发现了个体间Southern杂交带型改变的现象，尤其在丁_4的两个R_5家系中表现较复杂。The segregation, integration and stability of the transgene-Bt (Bacillus thuringensis)in eigth transformants created by three ways of transformation were studied by means of PCR (Polymerase chain reaction) and DNA molecular hybridization. The results are as follows:(1) The transgene-Bt in most of the progenies of the transformants showed simple Mendelian segregation. Although some deviation from Mendelian fashion existed in the early generations, the nounal Mendelian segregation would be found after R_3 generations and the transgene could fix in the populations. (2) The integration patterns of the transgene varied to the some extent in the different transformants, but they were characterized by either smaller band and copy numbers or cascade integration or closely linked integration in all transformants. (3) Changes of the Southern land pattem were found in some families of some transformants, especially, in the two families of Ding_4R_5.

关 键 词：玉米 BT毒蛋白基因 转基因 遗传 整合 

分 类 号：S513.035[农业科学—作物学]