H_2O_2对红细胞抗原性与LPO影响的FCM研究  

Studies on the Effect of H 2O 2 on the Antigenicity and LPO of RBC by FCM.

作　　者：符乃阳[1] 张尔贤[1] 

机构地区：[1]汕头大学理学院生物学系

出　　处：《生物化学与生物物理进展》1999年第3期254-258,共5页Progress In Biochemistry and Biophysics

摘　　要：用流式细胞计（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）测定Ｈ２Ｏ２损伤后的红细胞（ＲＢＣ）与ＩｇＧ的结合能力，并直接检测细胞自发荧光的变化，以研究Ｈ２Ｏ２对ＲＢＣ抗原性和脂质过氧化（ＬＰＯ）荧光产物生成的影响．结果表明ＲＢＣ抗原性和自发荧光变化与Ｈ２Ｏ２浓度和作用时间有关，抗原性变化对Ｈ２Ｏ２更敏感，比引起自发荧光明显增强所需Ｈ２Ｏ２浓度低两个数量级；Using flow cytometry(FCM) to measure IgG binding to red blood cells(RBC) exposed to H 2O 2 and their autofluorescence directly, in order to study the effect of H 2O 2 on the antigenicity and the production of fluorescent product of LPO of RBC. The results showed that the changes of both antigenicity and autofluorescence were dependent on the H 2O 2 concentration and the time of RBCs exposed to H 2O 2, and the change of antiginicity was more susceptible to H 2O 2 than that of autofluorescence . At 10 2 fold lower H 2O 2 concentration than that made autofluorescence significantly increase, IgG bond to RBC. Furthermore, it was found that the changes of antigenicity and autofluorescence were related to forward scatter (FSC).

关 键 词：红细胞 活性氧 细胞膜损伤 流式细胞计 抗原性 

分 类 号：R331.41[医药卫生—人体生理学] R556[医药卫生—基础医学]