蓖麻毒素A链突变体的设计、表达与活性研究  

The Modeling, Expression and Activity Study of Mutant Ricin Toxin A Chain.

作　　者：裴武红[1] 冯健男[1] 雷红星[1] 黎燕[1] 沈倍奋[1] 

机构地区：[1]军事医学科学院基础医学研究所

出　　处：《生物化学与生物物理进展》1999年第3期270-272,294,共4页Progress In Biochemistry and Biophysics

摘　　要：利用蛋白质结构同源模建并结合表观静电势分析，设计了拟具有生物学活性的蓖麻毒素Ａ链的突变体．将ＰＣＲ扩增的突变体基因，导入ｐＫＫ２２３３载体中，于大肠杆菌（Ｅ．ｃｏｌｉ）中获得高效、可溶性表达，而且。Combined protein homologous modeling with analysis of electrostatic surface potential energy, mutant ricin toxin A chain, which was suggested to be bioactive, was designed. The gene of ricin toxin A chain was amplified by PCR and inserted in pKK223 3 to construct a prokaryotic expression vector, which was highly expressed in E.coli system. Moreover, the expression products showed obvious cytotoxicity, just in agreement with the prediction of modeling.

关 键 词：蓖麻毒素 A链突变体 基因表达 分子设计 

分 类 号：R392.11[医药卫生—免疫学]