YY1抑制效应的破坏可促进人乳头瘤病毒16型癌基因的转录  

REMOVAL OF YY1'S REPRESSION EFFECT INCREASES THE TRANSCRIPTION OF THE ONCOGENE OF 16 HUMAN PAPILLOMAVIRUS 16

作　　者：董小平[1,2] 刘红[1,2] HerbertPfister 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]首都医科大学附属口腔医院

出　　处：《病毒学报》1999年第2期125-129,共5页Chinese Journal of Virology

摘　　要：人乳头瘤病毒１６型（ＨＰＶ１６）癌基因的表达受病毒早期启动子Ｐ９７的控制。位于ＬＣＲ上ＹＹ１蛋白结合位点的破坏可明显提高Ｐ９７的活性。为了观测ＹＹ１位点破坏在全基因组范围内对病毒ｅ６／ｅ７基因转录的影响，将构建的带有ＬＣＲ特异性突变的重组ＨＰＶ１６全基因组ＤＮＡ和ＨＰＶ１６野毒株ＤＮＡ转染至培养细胞，同时组建ＨＰＶ１６Ｅ６反向序列ＲＮＡ体外转录质粒。ＲＮａｓｅ保护试验证实，突变ＨＰＶ１６ＤＮＡ在短暂转染细胞中可诱导更多量的Ｅ６特异性ｍＲＮＡ转录。这一结果提示，ＬＣＲ上ＹＹ１位点的破坏可促进ＨＰＶ１６癌基因的转录，从而使感染细胞具有更高的癌变可能性。The expression of viral oncogenes of HPV 16 is controlled by the early promoter P97. Removal of YY1 binding sites in the long control regcon(LCR) can increase the activity of P97 in transient transfection assays. To analyse the effect on the transcription of viral oncogenes, due to destroying YY1's repression, we transfected HT3 cells separately with newly constructed HPV 16 DNAs containing mutated LCRs and the HPV 16 wild-type DNA. Meanwhile a subfragment of HPV 16 E6 antisense RNA was transcribed in vitro according to a newly generated plasmid. RNase protection assay showed that more E6 specific mRNAs could be detected under the control of the mutated HPV 16 DNAs. It suggests that removal of YY1 binding sequences in the LCR improves the transcription of viral oncogenens, thereby, might increase the viral oncogenicity.

关 键 词：人乳头瘤病毒 HPV YY1 癌基因 转录 

分 类 号：R373.9[医药卫生—病原生物学] R730.2[医药卫生—基础医学]