SPF级KM小鼠与BALB/c小鼠肠道总菌多样性的比较  

The comparison of the microflora diversity between SPF degree KM mice and BALB/c mice

作　　者：李伟[1,2] 唐欢[2] 周晓杨[2] 魏泓[2] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]第三军医大学基础部实验动物学教研室,重庆400038

出　　处：《中国微生态学杂志》2010年第10期865-867,875,共4页Chinese Journal of Microecology

基　　金：国家"973"计划(2007CB513007)

摘　　要：目的分析SPF级封闭群KM小鼠及近交系BALB/c小鼠的肠道菌群总菌多样性,比较两个不同遗传背景肠道总菌的丰富度、Shannon-Wiener指数和均匀度。方法收集SPF级KM小鼠和BALB/c小鼠新鲜粪便,提取粪便总菌DNA,用基于细菌16S rDNA序列的变性梯度凝胶电泳(PCR-DGGE)分析粪便总菌多样性。结果 SPF级KM小鼠及BALB/c小鼠粪便总菌多样性差异无统计学意义(P>0.05),品系内不同性别之间粪便总菌多样性差异亦无统计学意义(P>0.05)。结论选择SPF级小鼠进行微生态学相关研究时,封闭群KM小鼠及近交系BALB/c小鼠均可作为选择对象,同时可忽略菌群的性别差异。Objective To analyze the fecal microbiota of SPF KM mice and BALB/c mice,and compare the richness,Shannon-Wiener index and evenness of their total intestinal microflora.Method Fresh feces of SPF KM mice and BALB/c mice were collected.Total bacterial DNA of the fecal samples was extracted and 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE） was performed.Result No remarkable differences of diversity of total intestinal microflora were found between SPF KM mice and BALB/c mice（P〉0.05）,nor between different genders（P〉0.05）.Conclusion In the experiments related to the microbiota of SPF mice,KM mice and BALB/c mice were recommended equally and the difference of the gender could be neglected.

关 键 词：KM小鼠 BALB/C小鼠 菌群多样性 PCR—DGGE 

分 类 号：R378[医药卫生—病原生物学]