奶牛产后急性子宫内膜炎病原的分离与鉴定  

Isolation and identification on pathogenic microorganism from acute endometritis in postpartum dairy cows

作　　者：王国卿[1,2] 王洪海[3] 郭梦尧[2] 张乃生[2] 

机构地区：[1]东北大学生物技术研究所,辽宁沈阳110004 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2010年第10期10-12,共3页Chinese Journal of Veterinary Medicine

基　　金：中国博士后科研基金(20080440146);国家科技支撑课题"优质肉牛高效繁殖技术研究与应用"(3A4090016201)

摘　　要：通过研究奶牛产后急性子宫内膜炎致病微生物组成,揭示该病主要病原并为临床治疗提供依据。以产后6～10 d急性化脓性卡他性子宫内膜炎患牛80头为研究对象,对子宫内容物进行细菌和真菌的分离鉴定。共分离到195株细菌及真菌,其中35.4%具有溶血性,50.8%对试验动物有致病性;大肠杆菌和金黄色葡萄球菌分离率较高,为83.75%和62.5%,其次是奇异变形杆菌(36.25%)、白色念珠菌(20%)和肺炎克雷伯氏菌(17.5%)。该病的90%为混合感染,细菌混合感染(70%)主要由大肠杆菌、金黄色葡萄球菌、肺炎克雷伯氏菌和奇异变形杆菌组成;真菌与细菌混合感染占20%,均有白色念珠菌存在;细菌单一感染占10%。结果表明,奶牛产后急性子宫内膜炎主要致病菌是大肠杆菌、金黄色葡萄球菌、肺炎克雷伯氏菌、奇异变形杆菌,20%受试牛存在着以白色念珠菌为主的真菌感染。The compositions of acute endometritis pathogenic microorganism were studied in postpartum dairy cows in order to find the main pathogen and provide evidence for the clinical treatment.We had done some researches on 80 dairy cows which affected acute blennorrhea endometritis in postpartum 6～10 days,bacteria or fungi were isolated and identified from the contents of the uterus.195 bacterial or fungal isolates were obtained,35.4% were hemolytic and 50.8% were pathogenic to experimental animals.The most frequently bacterial isolates were E.coli and S.aureus（respectively 83.75% and 62.5%）,then P.mirabilis（36.25%）,C.albicans（20%） and K.pneumoniae（17.5%）.90% of the diseases were co-mixed infected,the bacterial co-mixed mixed infection conclude E.coli,S.aureus,K.pneumoniae and P.mirabilis（70%）,20% were fungi and bacterium co-infection,C.albicans were isolated.The results show that E.coli,S.aureus,K.pneumoniae,P.mirabilis were major pathogenic bacteria,20% dairy cows were infected with fungi,among which C.albicans were primary pathogens.

关 键 词：奶牛 急性子宫内膜炎 细菌 真菌 鉴定 

分 类 号：S858.23[农业科学—临床兽医学] S852.61[农业科学—兽医学]