基于冷热板示差法研究麻黄汤与麻杏石甘汤寒热药性差异  

Investigation of differences between Cold and Hot nature of Mahuang decoction and Maxing Shigan decoction based on cold/hot plate differentiating assay

作　　者：贾雷[1,2] 赵艳玲[1] 邢小燕[1,2] 王伽伯[1] 邹文俊[2] 李瑞生[3] 杨宏博[1,4] 程丹红[1,2] 肖小河[1] 

机构地区：[1]解放军第三○二医院全军中药研究所,北京100039 [2]成都中医药大学,四川成都610075 [3]解放军第三○二医院动物中心,北京100039 [4]昆明理工大学,云南昆明650224

出　　处：《中国中药杂志》2010年第20期2741-2744,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2007CB512607;2006CB504703)

摘　　要：目的：基于冷热板示差法研究麻黄汤与麻杏石甘汤寒热药性差异的客观真实性。方法：考察麻黄汤及麻杏石甘汤的给药剂量和测量时间,并进行验证性实验,测定ATPase等能量代谢指标。结果：给药剂量为10倍人体给药剂量（麻黄汤0.80 g.kg^-1,麻杏石甘汤2.26 g.kg^-1）,测量时间为给药后3060 min。与空白组比较,麻黄汤组小鼠在高温区停留比例明显降低（P〈0.01）;与麻黄汤组比较,麻杏石甘汤组小鼠在高温区停留比例显著增加（P〈0.05）。与空白组比较,麻黄汤组小鼠的Na^＋-K^＋-ATPase活性显著性增强（P〈0.05）,麻杏石甘汤组小鼠的Na^＋-K^＋-ATPase及Ca^2＋-Mg^2＋-ATPase活性均显著性降低（P〈0.01）。结论：从动物行为学角度验证了麻黄汤与麻杏石甘汤寒热药性差异的客观存在,结果与传统中医药理论对其寒热赋性具有一致性,肝组织ATP酶活性的改变可能是其作用机制。Objective： To investigate the objectivity and authenticity of the difference of the Cold and Hot nature between Mahuang decoction and Maxingshigan decoction based on the cold/hot plate differentiating assay.Method： The measuring time and dosage of Mahuang decoction and Maxingshigan decoction were investigated before the compliance experiment.Meanwhile,the activities of adenosine triphosphatase were detected in order to probe into the mechanism of their action.Result： It was found that the drug dose should be 10 times amount to human dose（0.80 g·kg^-1 of Mahuang decoction and 2.26 g·kg^-1 of Maxing Shigan decoction） and the measuring time should be from 30 min to 60 min after administering.Compared with the control group,the remaining ratio of the mice treated with Mahuang decoction on the hot pad significantly decreased（P〈0.01）.Compared with Mahuang decoction,the remaining ratio of the mice treated with Maxingshigan decoction on the hot pad significantly increased（P〈0.05）.Compared with the control group,the Na^＋-K^＋-ATPase activity of liver tissue of the mice treated by Mahuang decoction significantly increased（P〈0.05）,while the activity of Na^＋-K^＋-ATPase and Mg^2＋-Ca^2＋-ATPase of liver tissue of the mice treated by Maxingshigan decoction reduced significantly（P〈0.05）.Conclusion： This study showed that the differences of Cold and Hot nature of Mahuang decoction and Maxingshigan decoction were objectively existent from animal ethology,which was consistent with the traditional theroy of Chinese medicine.And the changes of ATPase activity of liver tissue might be the mechanism of drug action.

关 键 词：麻黄汤 麻杏石甘汤 寒热药性 冷热板示差法 

分 类 号：R289[医药卫生—方剂学]