HPLC测定丹皮中丹皮酚在大鼠体内血药浓度及药动学研究  

Determination of paeonol in rat plasma by HPLC and pharmacokinetic study

作　　者：陈晓兰[1,2] 陆洋[1] 杜守颖[1] 姚宗玲[1] 王珊[1] 李鹏跃[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]贵阳中医学院,贵州贵阳550002

出　　处：《中国中药杂志》2010年第21期2826-2828,共3页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项项目(2009ZX09502-008);教育部博士点基金项目(20090013110007)

摘　　要：目的：建立测定大鼠血浆中丹皮酚浓度的HPLC法,并用于丹皮酚注射给药后在大鼠体内的药动学研究。方法：采用水蒸汽蒸馏法提取丹皮中的丹皮酚,大鼠尾静脉注射4 mg.kg-1丹皮酚,于给药后不同时间采集血样,用乙腈处理血浆样品,震荡离心分离上清液用HPLC测定。选用DiamonsilTM钻石C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇-水（60∶40）,流速1.0 mL.min-1,检测波长274 nm,柱温常温,进样量20μL。结果：血浆样品中丹皮酚无干扰,丹皮酚在血浆样品中标准曲线线性范围0.20420.4 mg.L-1,r=0.999 9。丹皮酚平均回收率92.27%,最低检测浓度为10 mg.L-1,最低定量限LOQ为0.204 mg.L-1,日内、日间RSD〈4.9%。丹皮酚血药浓度数据用药动学软件kinetica处理,丹皮酚在大鼠体内的血药浓度-时间过程符合二房室模型,AUC=（111.88±14.44）mg·L^-1·min^-1,MRT=（23.25±5.86）min,Cmax=（8.99±0.84）mg.L-1,Kel=（0.082±0.015）min-1,t1/2 Kel=（8.73±1.54）min。结论：该方法简便、快速、重复性好,适用于丹皮酚大鼠血药浓度测定及药代动力学研究。Objective： To establish a sensitive HPLC method for determining the concentrations of paeonol in rat plasma and to evaluate its pharmacokinetic characteristics.Method： The paeonol from eortex Moutan was distilled by the way of water-vapor.A single iv dose of 4 mg·kg^-1 paeonol injection was given to 5 health rats.Paeonol was separated on a DiamonsilTM-C18 column with methanol-water（60∶40）as mobile phase.The plasma concentrationsof paeonol were determined and its pharmacokinetic parameters were calculated and evaluated by using kinetica 4.0.Result： The linear range of the method for paeonol was 0.204-20.4 mg·L^-1 and the determination limit was 0.204 mg·L^-1.The main pharmacokinetic parameters,such as AUC,MRT,Cmax,Kel,t1/2kel,after a single dose of paeonol injection were（111.88 ±14.44）mg·L^-1 ·min-1,（23.25 ±5.86） min,（8.99 ±0.84） mg·L^-1,（0.082 ±0.015） min-1 and（8.73 ±1.54） min,respectively.Conclusion： The HPLC method for determining paeonol concentration in plasma is simple,rapid,sensitive and suitable for pharmacokinetic studies.

关 键 词：丹皮酚 HPLC 血药浓度 药动学 

分 类 号：R285.5[医药卫生—中药学]