UPLC测定不同产地、不同部位小叶黑柴胡中黄酮含量  

Determination of flavones in different origin and parts of Bupleurum smithii var. parvifoliaum by UPLC-PDA

作　　者：汤芳玲[1,2] 蔡光明[1] 袁波[2] 张卓勇[3] 

机构地区：[1]中国人民解放军第三○二医院全军中药研究所,北京100039 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]首都师范大学,北京100048

出　　处：《中国中药杂志》2010年第21期2874-2876,共3页China Journal of Chinese Materia Medica

基　　金：北京市政府专项(0853279)

摘　　要：目的：建立一种超高效液相色谱法（UPLC）检测不同产地、不同部位小叶黑柴胡中黄酮含量的方法。方法：Acquity UPLC BEHTMC18色谱柱（2.1 mm×50 mm,1.7μm）,流动相为甲醇-0.1%H3PO4水梯度洗脱,流速0.4 mL.min-1,柱温30℃,检测波长为256 nm。结果：3个黄酮成分芦丁、槲皮素、异鼠李素分离良好,线性范围分别是0.1061.06μg,r=0.9998（n=6）;0.004 870.048 7μg,r=0.999 7（n=6）;0.022 00.220μg,r=0.999 8（n=6）。结论：该方法测定小叶黑柴胡中黄酮类成分的含量,具有简便准确。An ultra performance liquid chromatography method has been developed for determination of flavones in different origin and different parts from Bupleurum smithii var.parvifoliaum.The separation was performed at 30 ℃ on an Acquity UPLC BEHTM C18 column eluted with methanol and 0.1% phosphoric acid solution as mobile phases in gradient elution.The detection wavelength was set at 256 nm and the flow rate was 0.4 mL·min-1.There flavones of rutin,quercetin and isorhamnetin were separated well,the linear calibration curves were obtained over the ranges of 0.106-1.06 μg for rutin（r=0.999 8,n=6）,0.004 87-0.048 7 μg for quercetin（r=0.999 7,n=6）,0.022 0-0.220 μg for isorhamnetin（r=0.999 8,n=6）.The mean recoveries of the three compounds were 99.3%,97.8%,98.9% with RSD of 2.4%,3.0%,3.2%.The result showed that the method is convenient and feasible for determination of the flavone content in B.smithii var.parvifoliaum.

关 键 词：小叶黑柴胡 芦丁 槲皮素 异鼠李素 超高效液相色谱法 

分 类 号：R284[医药卫生—中药学]