四妙丸对大鼠佐剂性关节炎作用机制的研究  

Effects and mechanisms of Simiao pill on adjuvant arthritis rats model

作　　者：王晓玉[1] 张晓兰[1] 张丽[1] 李遇伯[1] 

机构地区：[1]天津中医药大学中药学院天津市中药化学与分析重点实验室,天津300193

出　　处：《中国中药杂志》2010年第21期2889-2892,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30801540)

摘　　要：目的:研究四妙丸对大鼠佐剂性关节炎(adjuvant arthritis,AA)的作用并初步探讨其作用机制,为类风湿关节炎的临床治疗提供实验依据。方法:Wistar大鼠,雄性,随机分为空白对照组(生理盐水)、模型对照组(生理盐水)、雷公藤多苷组(GTT9.45 mg.kg-1)、四妙丸低、中、高剂量组(1 080,2 160,4 320 mg.kg-1)。除空白组外,其余各组采用弗氏完全佐剂注射于大鼠右后足足跖皮内,建立佐剂性关节炎模型;空白组注射等量生理盐水,并分别给予灌胃治疗,观察各组足肿胀值,测定血清中SOD的含量,脾指数和胸腺指数,用sqRT-PCR法检测大鼠滑膜组织中细胞因子IL-1β,IL-6,TNF-αmRNA的表达。结果:四妙丸给药后,大鼠足肿胀均显著减轻,大鼠血清中SOD活性均显著提高,免疫器官脾脏肿大有所缓解,胸腺损害均显著减轻,滑膜组织中IL-1β,IL-6,TNF-αmRNA表达显著降低。结论:四妙丸对大鼠佐剂性关节炎有一定的治疗作用,可减轻炎症反应和关节肿胀,改善关节功能,对类风湿关节炎的临床治疗提供了可靠依据。Objective： To study pharmacologic actions for rheumatoid arthritis treatment and probe into the effects and mechanisms of Simiao pill on adjuvant arthritis（AA） rats.Method： Wistar rats,male,were randomly divided into the normal comparison group,model group,the glucosida Tripterygii TOTA group（9.45 mg·kg-1）,the Simiao pill low dose group（1 080 mg·kg-1）,the Simiao pill middle dose group（2 160 mg·kg-1） and the Simiao pill high dose group（4 320 mg·kg-1）.Except the normal comparison group,rats in the other groups were injected Freund′s complete adjuvant reagent into the rats′ right etapedes to establish the AA model.Drugs were given by intragastric administration.Then paw swelling,SOD in blood serum,the spleenand and thoracic gland index,histopathology change of malleolus joint were observed.IL-1β,IL-6 and TNF-α mRNA were observed by semi-quantitative reverse transcription and polymerase chain reaction（sqRT-PCR）.Result： Simiao pill could inhibit the swelling of the rats′ inflamed metapedes.Compared with the model group,each group of Simiao pill could increase SOD,decrease spleen index,advance the thoracic gland index and decrease IL-1β,IL-6 and TNF-α mRNA.Conclusion： Simiao pill could threat AA rats and inhibit the inflammation of synovial membrane obviously.It also could relieve the degree of paw swelling,and therefore provide clinical theatment basis of RA

关 键 词：四妙丸 佐剂性关节炎 作用机制 

分 类 号：R285.5[医药卫生—中药学]