通心络超微粉对大鼠损伤的内皮细胞骨架蛋白的影响  

Influence of Tongxinluo Superfine on Damaged Endothelial Cytoskeletal Protein in Rats

作　　者：高怀林[1] 王玲玲[1] 贾振华[1] 董小伟[1] 王宏涛[1] 刘克剑[1] 朱慧明[1] 吴以岭[1] 

机构地区：[1]河北医科大学附属以岭医院心脑血管络病国家中医药管理局重点研究室,石家庄市新石北路385号050091

出　　处：《中医杂志》2010年第10期931-933,共3页Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划("973"计划)资助项目(2005CB523301);国家"十一五"重大新药创制专项(2009ZX09313-003)

摘　　要：目的观察通心络超微粉(TXLU)对血管内皮功能的作用及机制。方法将健康雄性Wistar大鼠随机分为正常组和模型组,模型组以蛋氨酸饮食复合"基础饮食+负重游泳"法建立络气虚滞型血管内皮功能障碍动物模型。取正常组和模型组大鼠血清体外培养人脐静脉内皮细胞(HUVEC)细胞株,实验分为4组:空白组、正常血清组、模型血清组、TXLU组,每组4个复孔。观察血清作用HUVEC6h后细胞骨架蛋白纤维状肌动蛋白(F-actin)的形态、分布及含量变化。结果模型组血清作用6h后细胞骨架蛋白F-actin发生明显的破坏,其荧光强度减弱,TXLU预处理HUVEC后可减少F-actin损伤的发生及伴随含量的增加(P<0.01)。结论 TXLU可以减弱细胞骨架蛋白F-actin的损伤,可能是其改善血管内皮功能,防治动脉粥样硬化的机制之一。Objective To observe the influence of Tongxinluo Superfine （Submicron powder of Chinese medicines for preventing the atheroselerosis） on the endothelial cells and its mechanism. Methods Healthy male Wistar rats were randomized into normal group and model group. The model group was given a methionine diet plus basic diet and weight-loading swimming to establish the models of endothelial dysfunction in the collateral qi deficiency and stagnation syndrome. The serum of mice of the normal group and model group was used to culture in vitro the cell line of human umbilical vein endothelial cells （HUVEC） in an experiment of four groups, i.e. blank group, normal serum group, model serum group, and Tongxinluo Superfine group. The changes in the morphological structure and distribution and content of fibrous actin （F-actin） of endothelial cytoskeletal protein 6 hours after the serum functioned on HUVEC were observed. Results In the model group, a significant destruction of F-actin appeared after the serum functioning for 6 hours, and decreased fluorescence intensity existed. The preprocessing of HUVEC with Tongxinluo Superfine could reduce the incidence of damage of F-aetin and increase its content （P〈0.01）. Conclusion Tongxinluo Superfine can weaken the damage of F-actin of endothelial cytoskeletal protein, being part of the possible mechanism in improving the endothelial functions of vessels to prevent the atherosclerosis.

关 键 词：通心络超微粉 内皮功能 细胞骨架 络气虚滞 

分 类 号：R285.5[医药卫生—中药学]