超声介导微泡对内皮祖细胞一氧化氮及一氧化氮合酶活性的影响  

Effects of Ultrasound-mediated Microbubble on Nitric Oxide and Nitrogen Synthase of Endothelial Progenitor Cells

作　　者：魏红[1] 童嘉毅[2] 范国峰[3] 冯毅[2] 李鹏[2] 

机构地区：[1]东南大学医院,南京210018 [2]东南大学附属中大医院,南京2100092 [3]南京大学医学院附属鼓楼医院,南京210008

出　　处：《中国医科大学学报》2010年第10期806-808,共3页Journal of China Medical University

基　　金：高等学校博士学科点专项科研基金(20090092120059)

摘　　要：目的超声联合微泡能够显著增强干细胞移植治疗缺血性心血管疾病,但其作用机制仍不明确,本实验在体外对此进行初步探讨。方法体外提取、分离和培养猪骨髓内皮祖细胞,随机分为对照组、超声组、超声微泡组,干预90s后分别收集内皮祖细胞和细胞上清液。采用硝酸还原法检测上清液一氧化氮(NO)含量,分光光度计检测内皮祖细胞一氧化氮合酶的相对活力。结果超声微泡组内皮祖细胞上清液一氧化氮含量为(88.70±8.78)μmol/L,内皮祖细胞的诱导型一氧化氮合酶和结构型一氧化氮合酶活力分别为(10.81±0.95)U/ml、(4.78±0.36)U/ml,较对照组显著增加(P<0.05);超声组NO含量无明显改变,各型一氧化氮合酶活力较对照组有下降趋势,无显著差异。结论超声(1MHz,1W/cm2)介导微泡作用90s能够增强内皮祖细胞一氧化氮合酶活性,增加NO生成,从而增效干细胞治疗心肌梗死。Objective To explore the mechanism of ultrasound-mediated microbubbles on nitric oxide（NO）and nitrogen synthase（NOS） of endothelial progenitor cells.Methods Bone marrow-endothelial progenitor cells（EPCs）of Chinese minipigs were extracted,separated and cultured in vitro.The EPCs were divided into 3 groups：control group,ultrasound group and ultrasound-mediated microbubble group.After the EPCs suffered from intervene for 90 seconds,the NO concentration in the supernatant of EPCs and NOS relative activity were measured by nitrate reduction and fluorescent probe with spectroscopy respectively.Results Compared to the control group,NO concentration in the supernatant of EPCs and NOS relative activity of EPCs were enhanced in ultrasound-mediated microbubble group,but no change was found in ultrasound group.Conclusion 1 W/cm2 ultrasound-mediated microbubbles might enhance the effect of stem cells treatment for myocardial infarction through increasing NO generation and enhancing the NOS activity.

关 键 词：超声 超声微泡 内皮祖细胞 一氧化氮 一氧化氮合酶 

分 类 号：R233[医药卫生—中医学] R541.4