姜黄素对APP/PS1转基因鼠TNF-α、IL-6的影响  

Effects of Curcumin on Interleukin-6 and Tumor Necrosis Factor-α in APP/PS1 Transgenic Mice

作　　者：王铜浩[1] 杨茂伟[1] 颜培培[2] 初立伟[1] 高志达[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001 [2]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《中国医科大学学报》2010年第10期809-811,822,共4页Journal of China Medical University

基　　金：辽宁省教育厅高校科研基金资助项目(L2010590)

摘　　要：目的探讨APP/PS1转基因鼠体内细胞因子TNF-α和IL-6的变化规律以及不同药量的姜黄素对其变化规律的影响。方法 6月龄雌性小鼠分为:野生型小鼠(对照组,n=9),APP/PS1转基因鼠(模型组,n=9)和APP/PS1转基因鼠+姜黄素(用药组,n=27)。对照组和模型组自由摄食和饮水;用药组,姜黄素按60,160,600mg·kg-1加入小鼠饲料,各组小鼠每次取3只分别于9,12,18月龄时处死,应用ELISA检测其大脑、血清中的细胞因子TNF-α和IL-6。结果 (1)与野生型小鼠相比,APP/PS1转基因鼠脑组织、血清中的TNF-α和IL-6于9月龄时开始升高,12月龄时差异显著(P<0.05),18月龄时差异更加显著(P<0.01);(2)应用姜黄素60mg·kg-1治疗后,APP/PS1转基因鼠脑组织、血清中的TNF-α和IL-6表达水平降低,姜黄素160mg·kg-1出现显著性差异(P<0.05),姜黄素600mg·kg-1差异更加显著(P<0.01)。结论 APP/PS1转基因鼠随着病情发展脑组织、血清中TNF-α和IL-6水平逐渐升高,应用姜黄素治疗后细胞因子TNF-α和IL-6逐渐降低,并呈剂量依赖性。Objective To investigate the effects of different concentrations of curcumin on tumor necrosis factor-α（TNF-α）and interlukin6 （IL-6）in APP/PS1 transgenic mice.Methods A total of 45 female 6-month-old mice were divided into 3 groups：control group（wildtype mice,n =9）,model group（APP/PS1 transgenic mice,n =9）,and curcumin group（APP/PS1 transgenic mice treated with curcumin,n=27）.The rats of control group and model group were housed under ad libitum feeding conditions.The rats of Curcumin group were administrated with curcumin orally （60,160,600 mg·kg-1）.Three mice of each group were sacrifced at 9,12 and 18 months of age,and the brain and serum samples were harvested to analyse the levels of TNF-α and IL-6 by ELISA.Results Compared with the wild-type mice,the levels of TNF-α and IL-6 in the brain and serum of APP/PS1 transgenic mice began to increase from 9 months of age,and the significant differences were found at 12 and 18 months of age（P 〈 0.05,P 〈 0.01）.After the treatment of curcumin（60,160,600 mg·kg-1）,the levels of TNF-α and IL-6 in APP/PS1 transgenic mice degraded dose-dependently.Conclusion With the development of AD,the levels of TNF-α and IL-6 in the brain and serum of APP/PS1 transgenic mice increased gradually.After the treatment of curcumin,the levels of TNFα and IL-6 in APP/PS1 transgenic mice degraded dose-dependently.

关 键 词：姜黄素 APP/PS1转基因鼠 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R282.71[医药卫生—中药学]