高效液相色谱同时测定当归养血丸中3种成分的含量  

HPLC Simultaneous Determination of Three Ingredients in Danggui Yangxue Wan

作　　者：计耀成[1] 王玲[1] 曲京红[2] 李志浩[2] 

机构地区：[1]湖北丹江口市第一医院药学部,丹江口442700 [2]湖北郧阳医学院附属东风医院药剂部,十堰442008

出　　处：《中国医药导刊》2010年第10期1818-1819,1766,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:建立同时测定当归养血丸中芍药苷、白术内酯Ⅰ和白术内酯Ⅲ含量的方法。方法:采用HPLC法,ZORBAX-C18柱(4.6mm×250mm,5μm),流动相为乙腈(A)∶0.2%磷酸溶液(B),进行梯度洗脱(0-40min,A18%-62%,B82%-38%),流速为0.8ml·min^(-1),柱温:30℃,检测波长为222nm。结果:芍药苷、白术内酯Ⅰ和白术内酯Ⅲ分别在(0.014～0.276)mg·ml^(-1)(r=0.9996),(0.6～12.0)μg·ml^(-1)(r=0.9995)和(1.3～26.0)μg·ml^(-1)(r=0.9998)浓度范围内均有良好的线性关系,平均加样回收率分别为99.0%,98.0%,97.9%;RSD均小于2.0%。结论:本方法简便,准确,重复性好,可用于该制剂的质量控制。Objective：To establish a method for the determination of buddleoside, atractylenolide Ⅰ and atractylenolide III in Danggui Yangxue Wan.Methods：The HPLC system consisting the ZORBAX-C18（4.6mm×250mm,5μm）column,the mobile phase consisted of Acetonitrile（A）-0.2%H3PO4（B）with the gradient elution（0-40min,A18%-62%,B82%-38%）,the flow rate was 0.8ml.min^-1,the column temperature was 30℃,The UV detector was set at 222nm.Results：The linear response range was （0.014-0.276）ml.min^-1（r=0.9996）,（0.6-12：0）μg.ml^-1（r=0.9995）and （1.3-26.0）μg·ml^-1（r=0.9998）,respectively.The average recovery of buddleoside, atractylenolide Ⅰ and atractylenolide Ⅲ were 99.0%,98.0%,97.9%,respectively.Conclusion：The assay demonstrated that the method is simple, accurate and repeatable, it can be applied in quality control of Danggui Yangxue Wan.

关 键 词：芍药苷 白术内酯Ⅰ 白术内酯Ⅲ 当归养血丸 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]