补骨脂水煎液大鼠体内血清药物成分的初步研究  

Study on serum active constituents of rats intragastrically given decoction of fructus psoraleae

作　　者：宋殿荣[1] 宋红运[1] 王跃飞[2] 郭洁[1] 徐燕颖[1] 潘桂湘[2] 

机构地区：[1]天津中医药大学第二附属医院,天津300150 [2]天津中医药大学中医药研究院省部共建教育部方剂重点实验室,天津300193

出　　处：《中华中医药杂志》2010年第11期1863-1865,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技部中医药行业专项(No.200707011)~~

摘　　要：目的:对补骨脂水煎液进行血清药物化学分析,阐明其体内药效物质基础,为优化处方配伍提供实验依据。方法:建立补骨脂水煎液高效液相色谱(HPLC)指纹图谱,予大鼠补骨脂水煎液灌胃,分别于1、12h取血,通过比较空白血清、含药血清、补骨脂水煎液HPLC指纹图谱的差异,分析补骨脂水煎液的血清药物活性成分。结果:补骨脂水煎液中可检测到补骨脂苷、异补骨脂苷、补骨脂素、异补骨脂素和一未知的成分,灌服补骨脂水煎液后1、12h,大鼠血清中的移行成分均为补骨脂苷、异补骨脂苷、补骨脂素和异补骨脂素。结论:补骨脂苷、异补骨脂苷、补骨脂素及异补骨脂素是补骨脂水煎液入血的主要活性成分,是体内发挥药效的主要物质基础。Objective： To investigate the active constituents in decoction of Fructus Psoraleae by serum pharmacochemistry, and illuminate the elemental pharmacodynamic constituents, and provide the foundation for optimizing the prescription of fructus psoraleae. Methods： Based on the establishment of HPLC fingerprint of decoction of Fructus Psoraleae, rat serum were obtained after being given with decoction of Fructus Psoraleae 1h and 12h, compared HPLC fingerprints of blank serum, serum containing drug and decoction of Fructus Psoraleae, and the serum active constituents in decoction of Fructus Psoraleae were analyzed. Results： Psoralenoside, isopsoralenoside, psoralen and isopsoralen and the unknown constituent NO.3 chromatographic peak were determined in the decoction of Fructus Psoraleae. Psoralenoside, isopsoralenoside, psoralen and isopsoralen were determined in the rat serum after being intragastrically administrated decoction of Fructus Psoraleae 1h and12h. Conclusion： Psoralenoside, isopsoralenoside, psoralen and isopsoralen were the main active constituents and the elemental pharmacodynamic constituents of the decoction of Fructus Psoraleae.

关 键 词：补骨脂水煎液 补骨脂苷 异补骨脂苷 补骨脂素 异补骨脂素 高效液相色谱 指纹图谱 

分 类 号：R285.5[医药卫生—中药学]