基于血清蛋白组学的慢性肾衰中医湿证鉴别证型探讨  

Exporing on differential diagnosis model of damp-syndrome of TCM in chronic renal failure based on serum proteomics

作　　者：何磊[1] 程亚伟[2] 廖萍[3] 胡衡[3] 金亚明[4] 李福凤[2] 王文静[3] 王忆勤[2] 

机构地区：[1]河南中医学院中医诊断学学科,郑州450008 [2]上海中医药大学中医证实验室,上海201203 [3]上海市疾病预防控制中心公共卫生分子生物学研究室,上海200336 [4]上海中医药大学附属龙华医院肾内科,上海200032

出　　处：《中华中医药杂志》2010年第11期1892-1894,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市教委科研创新项目(No.08ZZ63);上海市第三期重点学科经费资助项目(No.S30302)~~

摘　　要：目的:通过分析慢性肾功能衰竭中医湿证与非湿证的血清蛋白质谱差异,筛选血清蛋白标志物并建立湿证诊断鉴别证型,探讨其在慢性肾衰湿证血清学诊断中的意义。方法:收集56例慢性肾衰湿证患者和27例非湿证患者的血清,经表面增强激光解析离子化飞行时间质谱(SELDI-TOF-MS)检验并筛选血清蛋白标志物。经生物信息学分析建立预测证型并进行验证。结果:在质荷比(M/Z)1500-30000范围内,检测到64个有效蛋白峰,发现有6个峰有显著差异(P<0.01),湿证组表达较低;主成分分析显示,组内样本彼此靠近,组间样本彼此分开。构建的湿证鉴别诊断证型,预测正确率为83.13%,灵敏度为82.14%,特异度为85.19%。结论:该证型能对慢性肾衰湿证做出较为准确的鉴别判断,为慢性肾衰湿证的临床诊断、辨证及治疗提供一定的实验依据。Objective ：To screen serum protein markers related to damp-syndrome of TCM in CRF and establish the differential diagnosis model of damp-syndrome, exploring and discussing its significance in serodiagnosis by comparing differences of serum protein spectrum expression between patients with damp-syndrome of TCM and ones with non-damp- syndrome of TCM. Methods： Collecting 56 patients with damp syndrome and 27 ones with non-damp-syndrome of TCM in CRF. Serum samples were tested by surface enhanced laser desorption/ionization-time of flight-mass spectrometry （SELDI-TOF-MS）. The data were analyzed to screen serum proteomic biomarkers. By bioinformatics analysis, differential diagnosis models were to be established and tested. Results： A total of 6 effective protein peaks were significantly different between damp-syndrome of TCM group and non-damp-syndrome of TCM group （P0.01） at m/z range of 1500 to 30000, among which showed low expression in damp-syndrome of TCM group. Damp-syndrome group and non-damp-syndrome group was obviously different from the nature of the clustering; and samples of each group near each other, inter-group samples from each other. By the bioinformatics analysis, establishing a differential diagnosis model of damp-syndrome of TCM, which was 83.13% in prediction accuracy rate with a sensitivity of 82.14% and a specificity of 85.19%. Conclusion： Differential diagnosis model made more accurate differential judgments and provided the experimental evidence for clinical diagnosis, differentiation and treatment of damp-syndrome of TCM in CRF.

关 键 词：慢性肾功能衰竭 湿证 CM10蛋白芯片 血清 

分 类 号：R277.5[医药卫生—中医学]