藻黄合剂对高糖环境人肾小球系膜细胞分泌MMP-9及TIMP-1的影响  

Effect of Zaohuang Misture on expressions of matrixmetalloproteinase-9 and tissue inhibitor of metalloproteinases-1 of human glomerular mesangial cells cultured under high glucose environment

作　　者：牟授菡[1] 尹燕志[1] 鞠建伟[1] 吕丛奎[1] 张李梅[2] 

机构地区：[1]烟台经济技术开发区医院,烟台264006 [2]滨州医学院,滨州256603

出　　处：《中华中医药杂志》2010年第11期1898-1900,共3页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:研究藻黄合剂对体外培养人肾小球系膜细胞(GMC)在高糖下分泌MMP-9、TIMP-1的影响。方法:体外培养人肾小球系膜细胞并在高糖培养基中孵育,用含有不同浓度的藻黄合剂培养液进行干预,分别观察24、48h系膜细胞MMP-9和TIMP-1的分泌。结果:藻黄合剂各剂量组在24、48h均显著升高髙糖下MMP-9的分泌(P<0.01);同时降低TIMP-1在48h的分泌(P<0.05,P<0.01)。结论:藻黄合剂可以升高高糖下系膜细胞MMP-9分泌,降低TIMP-1的分泌,这可能是藻黄合剂降解细胞外基质、防治糖尿病肾病的作用机制之一。Objective： To investigate the effect of Zaohuang Misture on expression of matrixmetalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinases-1（TIMP-1） by human glomerular mesangial cells （GMCs） cultured under high - glucose environment. Methods： The culture of GMCs was continued under high glucose before the experiment. The changes of MMP-9 and TIMP-1 in the GMCs and in Zaohuang Misture group were examined at 24h, 48h. Results： Compared with the high glucose group, the expression of MMP-9 in Zaohuang Misture were significantly higher at 24h, 48h （P0.01）; the expression of TIMP -1 in Zaohuang Misture were significantly lower at 48h （P0.05 or P0.01）. Conclusion： Zaohuang Misture may modulate the process of diabetic nephropathy by changing the expression of MMP-9 and TIMP-1.

关 键 词：藻黄合剂 金属基质蛋白酶-9 金属蛋白酶组织抑制剂-1 人肾小球系膜细胞 高糖 

分 类 号：R285[医药卫生—中药学]