三七总皂甙对实验性骨折愈合过程中低氧诱导因子-1αmRNA表达的影响  

Effects of Total Saponins of Panax Notoginseng on Expression of Hypoxia Inducible Factor-1αduring Fracture Healing

作　　者：刘东宁 刘广明[2] 易伟宏 王锡三 王尔天 王敏 黄曹 刘赫 李学东[3] 

机构地区：[1]深圳第六人民医院脊柱外科,广东深圳518052 [2]北京公安医院病理科 [3]汕头大学医学院第一附属医院骨科

出　　处：《中国伤残医学》2010年第5期7-9,共3页Chinese Journal of Trauma and Disability Medicine

基　　金：2010年深圳市科技计划一般支助项目(201002172)

摘　　要：目的:探讨低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)在骨折愈合过程中的表达及三七总皂甙(total saponins of panax notoginseng,PNS)的干预作用。方法:在36只雄性SD大鼠左桡骨形成骨折模型,后随机分成对照组和PNS组,予以PNS100mg/kg/d腹腔内注射,对照组予以等量盐水。在骨折后7,14,21天X线观察骨折愈合及骨痂形成情况;HE染色了解骨痂形成情况;RT-PCR方法检测骨痂组织HIF-1αmRNA的表达。结果:X线显示PNS组骨痂形成明显加快,HE染色显示骨折断端成骨细胞数目及骨痂增多;对照组HIF-1αmRNA7天表达较低、14天达峰值、21天下降;PNS组7天、14天时点表达高于对照组(P<0.05),21天低于对照组P<0.05)。结论:PNS可上调骨折愈合过程中骨痂组织HIF-1α的表达,改善骨折部位的血供,促进骨折愈合。Objective：To investigate the effects of total saponins of panax notoginseng on the expression of hypoxia inducible factor-1αduring fracture healing.Methods：36 rats were divided into PNS group and control group,the left radius fracture model were used in this experiment.PNS 100mg/kg/d were intraperitoneal injections in rats of PNS groups and same dose isotonic saline were treated in control group as PNS.Rats in control group and PNS group were sacrificed and necropsied at 7d,14d and 21d （n=6）.The tissue or callus around fracture site were obtained for histological examnation and callus measurement.The formation of callus were monitored by X-ray.The expression of HIF-1α mRNA of callus during fracture healing were detected by RT-PCR.Results：The formation and thickness of callus were enhanced by PNS; HE staining showed that the chondrocytes,osteoblasts and callus appeared earlier and more in PNS groups than control （p0.05）.The quantity of HIF-1α mRNA in the callus of PNS groups had a significant increase at 7d,14d time point（p0.05）and significant decrease at 21d compared with control group.Conclusion：PNS could increase callus content and quantity of HIF-1α mRNA in the callus.To increase the expression of HIF-1α might be the potential mechanism of PNS on accelerate the fracture healing.

关 键 词：三七总皂甙 骨折 骨痂 低氧诱导因子1Α 

分 类 号：R683[医药卫生—骨科学]