中药材丹参中4种主要丹参酮的超高效液相色谱法测定研究  

The Determination of Tanshinones in Salvia Miltiorrhiza Bge by Ultra Performance Liquid Chromatography

作　　者：母继林[1,2] 和顺琴[1] 薛景娇[1] 曹靖丽[1] 胡秋芬[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031 [2]临沧地区永徳县人民医院,云南临沧677600

出　　处：《云南民族大学学报（自然科学版）》2010年第6期407-409,共3页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(20802082);民族药资源化学国家民委-教育部重点实验室基金(MJY090102)

摘　　要：建立了用超高效液相色谱法（UPLC）同时测定中药材丹参中隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA和二氢丹参酮的超高效液相色谱法.中药材样品中的丹参酮用高速匀浆法提取,采用Waters ACQUITY UPLC BEHC18（1.7μm,2.1 mm×50 mm）超高效液相色谱柱为固定相,40%的乙腈（内含0.1%的磷酸）为流动相分离,检测波长为254 nm,流速为0.6 mL/min.在上述色谱条件下隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA和二氢丹参酮在5.0 min内实现基线分离,加标回收率在91.5%~98.6%之间,日内相对标准偏差为2.1%,日间相对标准偏差为2.8%,结果满意.A new method for the determination of tanshinones in salvia miltiorrhiza bge by ultra performance lquid chromatography（UPLC） was studied.The tanshinones were extracted from the samples by high speed homogenization with acetonitrile.Then,the tanshinones were separated on Waters ACQUITY UPLC BEH C18（1.7 μm,2.1×50 mm） column with 40% acetonitrile（containing 0.1% phosphoric acid） as the mobile phase at 0.6 ml/min.The detect wavelength was 254 nm.Under the optimal conditions,four tanshinones（dihydrotanshinone,crypto tanshinone,tanshinone I,tanshinone ⅡA） can achieve a baseline separation within 5.0 min.The relative standard derivation of overall intra-day variations was 2.1%,and the relative standard derivation of inter-day variations was 2.8%.The standard recoveries（three different concentrations of markers） were ranged from 91.5%~98.6%.The results are satisfactory.

关 键 词：丹参酮 丹参 超高效液相色谱法 

分 类 号：O657.7[理学—分析化学]