羔羊皱胃酶的两种盐析效果比较研究  

COMPARISON OF SALTING-OUT EFFECTS OF LAMB RENNET IN TWO SALTING-OUT AGENTS

作　　者：宋宏新[1] 马瑛东[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《河南工业大学学报（自然科学版）》2010年第5期38-41,共4页Journal of Henan University of Technology：Natural Science Edition

摘　　要：采用NaCl和(NH4)2SO4两种盐析剂在pH4.6、4℃下对羔羊皱胃进行盐析,盐析时间为14 h,比较两种不同的盐析剂的盐析效果.分析经不同饱和度的盐溶液盐析沉淀得到的皱胃酶蛋白含量、活性回收率和纯化效果.结果表明:酶活性回收率较高的为(NH4)2SO4,其活性回收率为90%,而纯化效果最好的为NaCl,可使皱胃酶纯化2.13倍.In this paper,we studied and compared the salting-out effects of lamb rennet in two salting-out agents,i.e.NaCl and（NH4）2SO4,under the conditions of pH 4.6,temperature 4 ℃,and salting-out time 14 hours.We also analyzed the protein content,the activity recovery and the purification effect of the rennet obtained by salting out in salt solutions with different saturations.The results showed that the rennet had higher activity recovery of 90% in the（NH4）2SO4 salting-out agent;and the rennet had higher purification factor of 2.13 in the NaCl salting-out agent.

关 键 词：羔羊皱胃酶 酶活性回收率 纯化倍数 

分 类 号：TS201.2[轻工技术与工程—食品科学]