通脉注射液对脑缺血再灌注大鼠大脑皮质神经细胞N-甲基-D-天冬氨酸受体1 mRNA表达的影响  

Influence of Tongmai Injection on N-methyl-D-aspartate Receptor 1 mRNA Expression in Cortex Neurons of Rats with Cerebral Ischemia/Reperfusion

作　　者：朱兆洪[1] 丁柱[1] 刘茂才[2] 

机构地区：[1]解放军第421医院,广东广州510318 [2]广东省中医院,广东广州510120

出　　处：《广州中医药大学学报》2010年第6期606-608,613,666,共5页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】观察通脉注射液对脑缺血再灌注大鼠大脑皮质神经细胞N-甲基-D-天冬氨酸受体1(NMDAR1)mRNA表达的影响。【方法】选用SD大鼠随机分为假手术组,模型组,通脉注射液高、中、低剂量组(中药高、中、低剂量组,剂量分别为2.72、1.36、0.68 g.kg-1.d-1)。除假手术组外,其他组均采用改良4血管结扎法复制大鼠全脑缺血模型,取各组大鼠大脑皮质,采用原位杂交法检测各组大脑皮质神经细胞NMDAR1 mRNA阳性细胞数和染色光密度(D)值。【结果】与假手术组比较,模型组大鼠大脑皮质神经细胞NMDAR1 mRNA阳性细胞数和D值均显著增高(P<0.01或P<0.001);与模型组比较,中药高、中剂量组阳性细胞数显著减少(P<0.01或P<0.001),中药高剂量组的D值显著降低(P<0.01),而中药中、低剂量组的D值和中药低剂量组的阳性细胞数无显著变化(P>0.05)。【结论】通脉注射液治疗缺血性中风的作用与其能抑制缺血后神经细胞NMDAR1 mRNA的异常表达,减弱兴奋性神经毒性引发的神经细胞损伤有关。Objective To observe the influence of （NMDARI） mRNA expression of cortex neuron in Tongmai Injection （TI） on N-methyl-D-aspartate receptor 1 rats with cerebral ischemia/repeKusion. Methods SD rats were randomized into pseudo-operation group, model group, and high-, moderate- and low-dosage TI groups （2.72, 1.36 and 0.68 g·kg^-·d^-1 respectively）. Rat models with cerebral ischemia/reperfusion were established by four-vessel-occlusion method. Expression of NMDAR1 mRNA in cortex neuron and the staining optical density （D） value in alt of the groups were examined by in-situ hybridization. Results The number of cortex neurons with positive NMDAR1 mRNA expression and D value were increased in the model group （P 〈0.01 or P 〈 0. 001 compared with those in the pseudo-operation group ）. Compared with the model group, the number of cortex neurons with positive NMDAR1 mRNA expression was increased in high- and moderate-dosage TI groups （P 〈 0.01 or P 〈0. 001 ） , and D value was reduced in high-dosage TI group （P 〈0. 01 ）. NMDAR1 mRNA expression and D value remained unchanged in other TI groups （P 〉 0. 05 ）. Conclusion The therapeutic mechanism of TI for ischemic stroke is probably related with the inhibition of abnormal NMDAR1 mRNA expression in cortex neurons after cerebral ischemia/reperfusion, and with the reduction of neuronal injury induced by excitability neurotoxic effect.

关 键 词：通脉注射液/药理学 缺血性中风/中药疗法 脑/病理学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]