通过玉米茎尖转化耐草甘膦基因2mG2-epsps及抗性鉴定  

Transformation of Tolerant-gyphosate 2mG2-epsps Gene by Maize Shoot Tip and Identification of Resistance

作　　者：余桂容[1,2] 杜文平[2] 宋军[2] 窦茜[2] 刘永胜[1] 陆伟[3] 徐利远[2] 

机构地区：[1]四川大学生命科学院,四川成都610064 [2]四川省农业科学院生物技术核技术研究所,四川成都610066 [3]中国农业科学院生物技术研究所,北京100081

出　　处：《西南农业学报》2010年第5期1403-1408,共6页Southwest China Journal of Agricultural Sciences

基　　金：转基因生物新品种培育科技重大专项课题(2009ZX08003-003B转新型高抗草甘膦基因(2mG2-epsps)玉米新组合选育);四川省农科院优秀论文基金项目(2009-2010转耐草甘膦兼抗虫玉米研究);四川省应用基础项目(07JY029-103-5)

摘　　要：以X2211、78599等10个优良玉米自交系的茎尖为受体材料,采用农杆菌介导和真空负压法将抗除草剂基因2mG2-epsps转入玉米茎尖生长点分生组织细胞,转化后的茎尖经共培养3 d,植入花盆,3叶1心经除草剂（glyphosate,草甘膦）筛选,产生耐草甘膦1‰~1.25‰植株,移栽大田。从种子萌发准备茎尖到转化苗喷施除草剂筛选、移栽大田,整个周期只需30 d左右。同时,经特异PCR检测表明：2mG2-epsps基因已整合进部分植株。10 elite maize inbred lines , such as X2211 and 78599, their shoot tip meristematic cell were thansfered via Agrobacterium with plasmid construct that contained 2mG2-epsps gene under vacuum negative pressure. The transgenic plants were co-cultured for 3 days,then planted in flowerpot,selected by glyphosate at 3 leaves and I heart-leaf per/od and tolerant-glyphosate （ 1‰ - 1.25 ‰） plants were and then, planted out to field. From seed germination to tolerant-plant planted out, the whole time was about 30 days. At the same time, the allelespecific PCR analysis demonstrated that the 2mG2-epsps gene was transformed into the maize genome in some transgenic plants.

关 键 词：玉米茎尖 农杆菌真空转化 草甘膦 2mG2-epsps基因 

分 类 号：S513[农业科学—作物学]