纽荷尔脐橙及其果形变异体的AFLP分析  

Identification of Newhall Navel Orange and Its Fruit Shape Variants by AFLP

作　　者：彭志军[1] 李金强[1] 蔡永强[1] 陈守一[1] 郭文武[2] 

机构地区：[1]贵州省农业科学院果树科学研究所,贵州贵阳550006 [2]华中农业大学作物遗传改良国家重点实验室,湖北武汉430070

出　　处：《西南农业学报》2010年第5期1636-1639,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家863计划项目子课题"柑橘分子育种技术与品种创制"(2006AA100108);贵州省农业动植物育种专项课题"长圆形纽荷尔脐橙变异株分子标记鉴定及栽培利用研究"[黔农育专字(2007)023]

摘　　要：为揭示荷尔脐橙及其果形变异体在DNA水平上的差异,采用AFLP分子标记,从120对EcoRI/MseI酶切引物中筛选出具有多态性的3个引物对,对纽荷尔脐橙(Citrus sinensisL.Osbeck)及其果形变异体进行了扩增,共扩增出138条带,其中4条具有多态性,多态性比例占2.79%。利用遗传多样性分析软件NTSYSpc 2.1软件进行统计分析,得到品种(系)间的遗传距离为0.007353 0。说明,长圆形纽荷尔脐橙的形状特异性受遗传物质的控制,而不是受环境影响产生的变异。Three primer pairs with polymorphism selected from 120 pairs of primers of Newhall navel orange and its fruit shape variants were amplified by AFLP to determine their genetic difference on DNA level. The results showed that four polymorphic bands were identified from 138 amplified bands and the polymorphic ratio was 2.79 %. The genetic distance and similarity coefficient between two variants analyzed by NTSYSpe was 0. 007 353 and 0. 992 647, which indicates that the character of Newhall navel orange with oval fruit shape was dominated by DNA. , not by environment.

关 键 词：AFLP分析 纽荷尔脐橙 遗传距离 

分 类 号：S666.4[农业科学—果树学]