灰葡萄孢(Botrytis cinerea)对苯醚甲环唑的敏感性及其对不同杀菌剂的交互抗药性  

The Sensitivity of Botrytis cinerea to Difenoconazole and Cross-resistance against Diverse Fungicides

作　　者：赵建江[1,2] 韩秀英[1,2] 张小风[1,2] 马志强[1,2] 王文桥[1,2] 

机构地区：[1]河北省农林科学院植物保护研究所,河北保定071000 [2]河北省农业有害生物综合防治工程技术研究中心,河北保定071000

出　　处：《中国农学通报》2010年第22期282-286,共5页Chinese Agricultural Science Bulletin

基　　金：农业公益性行业科研专项(200903033);"十一五"国家科技支撑计划项目(2006BAD08A03);河北省科技支撑计划项目(06220110D)

摘　　要：测定山东和河北番茄主产区的灰葡萄孢对苯醚甲环唑的敏感性状况,并测定苯醚甲环唑与常规杀菌剂之间是否存在交互抗性;采用菌丝生长速率法测定灰葡萄孢对苯醚甲环唑的敏感性,通过分析苯醚甲环唑毒力对数与供试药剂毒力对数的相关性,判断苯醚甲环唑与各药剂之间是否存在交互抗性;被测定的86株野生菌株对苯醚甲环唑的敏感性(EC50值)在0.0135～1.2832μg/mL之间,相差95.05倍,平均值为(0.3958±0.3485)μg/mL,可作为敏感基线。田间已出现对苯醚甲环唑的低抗菌株,占检测总株数的9.5%,未检测到中抗菌株和高抗菌株。苯醚甲环唑EC50对数值与嘧霉胺、异菌脲、腐霉利、多菌灵和乙霉威的EC50对数值之间的相关系数分别为0.0192、0.0253、0.0053、0.0146和0.0567,表明苯醚甲环唑与供试药剂之间不存在交互抗性;苯醚甲环唑对灰葡萄孢的活性很高,并且与嘧霉胺、异菌脲、腐霉利、多菌灵和乙霉威之间不存在交互抗性,有望成为防治灰霉病的替代药剂。The Sensitivity of Botrytis cinerea to difenoconazole was tested in Shandong and Hebei provinces of main tomato production and cross-resistances of difenoconazole with botryticides were also examined;The sensitivity of Botrytis cinerea to difenoconazole was tested by measuring mycelial growth rate and cross-risistances of difenoconazole with botryticides were examined through analysing the correlation coefficent of the logarithms of that EC 50 of difenoconazole and botryticides to B.cinerea.EC 50 values for 86 isolates from the areas where the difenoconazole have never been used before were （0.3958 ± 0.3485） μg/mL,ranging from 0.0135μg/mL to 1.2832 μg/mL.The data could be used as baseline-sensitivity of B.cinerea to difenoconazole.The results of monitoring showed that low resistant isolates to difenoconazole in B.cinerea occurred in collected areas,9.5% low resistant isolates existed,but moderate resistant or high resistant isolates were not detected.The correlation coefficent of the logarithms of that EC 50 values between difenoconazole and other fungicides that pyrimethanil,iprodione,procymidone,carbendazim,and diethofencarb to B.cinerea were 0.0192、0.0253、0.0053、0.0146 and 0.0567.The results indicated that there were no positively-correlated cross-resistance between difenoconazole and other diverse active mechanism fungicides.Difenoconazole was expected to be a substituted pesticide in controlling gray mold,duing to difenoconazole had highly activity against Botrytis cinerea and there were no positively-correlated cross resistance between difenoconazole and other diverse active mechanism fungicides

关 键 词：灰葡萄孢 苯醚甲环唑 敏感基线 交互抗性 

分 类 号：S481.4[农业科学—农药学]