胰岛素样生长因子-I及其受体在糖尿病足溃疡的表达及意义  

Characteristics of insulin-like growth factor- I and receptor expression in Diabetic Foot

作　　者：秦庆新[1] 肖正华[1] 谢晓斌[1] 

机构地区：[1]广州市第一人民医院内分泌内科,510180

出　　处：《中国临床实用医学》2010年第11期40-43,共4页China Clinical Practical Medicine

摘　　要：目的探讨胰岛素样生长因子-I（Insulin—likegrowthfactor-I，IGF-I）与其受体两亚基（α，B）在非糖尿病正常皮肤和单纯糖尿病患者皮肤，以及糖尿病足患者足溃疡创面中的分布和表达特征，旨在阐明其表达水平变化与糖尿病足溃疡创面的内在联系。方法按照病例入选标准收集30例糖尿病足患者，23例单纯糖尿病患者，以及25例非糖尿病正常人，取足部皮肤组织标本，经固定脱水，石蜡包埋，切片后备用。采用免疫组织化学SP法检测所有取材组织中IGF-I、IGF-IRα和IGF-IRB蛋白定位及含量。在400倍光镜下观察免疫组化结果。结果在非糖尿病正常皮肤和单纯糖尿病患者皮肤，以及糖尿病足溃疡创面皮肤中IGF—I、IGF—I Rα和IGF—IRβ都有阳性表达，但变化规律不尽相同。IGF—I的阳性细胞率单纯糖尿病组较正常对照组表达减弱（P〉0．05），糖尿病足溃疡组较单纯糖尿病组表达进一步减弱（P〉0．05）。IGF—IRα和IGF—IRβ的阳性细胞率单纯糖尿病组较对照组表达减弱（P〉0．05），糖尿病足溃疡组较单纯糖尿病组表达进一步减弱（P〉0．05）。结论糖尿病患者创面IGF-I的缺乏或不足可能与糖尿病所引发的内皮细胞损害有关，与糖尿病足部溃疡经久不愈有直接关系。创面IGF-IRα和IGF—IRβ表达减少，和生物活性的降低，影响细胞因子引起的信号传递和核内目的基因表达，导致创面修复失控形成慢性难愈性溃疡。Objective This study investigated the distribution and expression of the IGF- I and its receptor 2 subunits（ α,β）in non-diabetic and diabetic patients＇ normal skin as well as diabetic foot-ulcer, to clarify the expression of level change and diabetic foot ulcer inherent connection. Methods the serum and foot skin of diabetes foot patients （ 30 cases） , diabetes patients （ 23 cases） and control （ 25 cases） ware collected. After fixation,dehydration, embedding, slicing, the line IGF-1, IGF-I Rαand IGF-I Rβimmunohistochemistry. observed the results of immunohischemistry under light microscope at 400 times. Results IGF- ,IGF- I Rαand IGF- I Rβis positive in non-diabetic or diabetic patients＇ normal skin and diabetic foot ulcer,but the variation is different. IGF- I positive cell rate of DM group than in normal control group decreased （ P 〉 0. 05 ） , Diabetic foot ulcer group than in the expression of further weakening in simple diabetic group （ P 〉 0. 05 ）. IGF- I Rαand IGF- I Rβpositive cell rate of DM group than in normal control group decreased （P 〉 0. 05 ） , Diabetic foot ulcer group than in the expression of further weakening in simple diabetic group （ P 〉 0. 05 ）. Conclusion IGF- I diabetic wound the lack or insufficiency may be caused by diabetes, endothelial cell damage, and prolonged healing of diabetic foot ulcers are directly related. Wound expression of IGF- I Rαand IGF- I Rβ decreased, and biological activity of the reduced impact of cytokine induced signaling and gene expression in the nucleus, resulting in lost control and wound healing of chronic dermal ulcer formation.

关 键 词：胰岛素样生长因子-I 胰岛素样生长因子-I受体 糖尿病足溃疡 

分 类 号：R587.2[医药卫生—内分泌]