利用AFLP技术分析丹顶鹤的亲缘关系  

Analysis on Relationship of the Red-crowned Cranes by AFLP

作　　者：李淑玲[1] 计子娟[2] 王宇[2] 

机构地区：[1]东北农业大学成栋学院生物食品系,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《动物学杂志》2010年第5期141-148,共8页Chinese Journal of Zoology

基　　金：黑龙江省自然科学基金项目(No.C200921)

摘　　要：建立了丹顶鹤(Grus japonensis)AFLP分析体系,经筛选,利用28对选择性扩增引物构建了5对丹顶鹤AFLP亲缘关系分析图谱,共得到1 114个扩增条带,其中多态性条带551条,多态性比例为49.5%。每个引物组合扩增的条带数为20～66条,其中,引物E4M1扩增的条带最多,为66条;引物E6M1扩增的条带数最少,为20条。经统计分析,计算了各样品间的相似性系数在0.71～0.88之间,得到5对丹顶鹤的遗传距离,并构建了UPGMA聚类图,结果1号与2号、3号与4号鹤的亲缘关系较近,其余3对鹤(自然配对)亲缘关系较远。表明丹顶鹤具有识别亲缘关系的行为机制,丰富了丹顶鹤繁殖行为机制的研究内容,并为深入研究建立合理的散养丹顶鹤繁育体系提出了建议。The analytical system was constructed for the amplified fragment length polymorphism（AFLP） in Red-crowned Crane（Grus japonensis）.The 5 couples of Red-crowned Crane were scanned by AFLP marker using the selective 28 primer combinations.The polymorphism and genetic cluster graph were acquired applying 10 individuals belonging to the 5 couples of Red-crowned Crane.A total of 551 polymorphism sites of the total of 1 114 bands were amplified from them.The percentage of polymorphism is 49.5%,each primer couples made 20-66 polymorphism sites.Primer E4M1 made the most,66 polymorphism sites;and primer E6M1 made the least,20 polymorphism sites.The similarity coefficient between the 10 Redcrowned Cranes was calculated,which were all in 0.71-0.88.The genetic distance and the UPGMA tree of 5 couples of Red-crowned Crane had been acquired.Cluster analysis showed that No.1 had close relationship with No.2;No.3 had also very close relationship with No.4;and the other 3 couple cranes had far genetic relationship.These showed that Red-crowned Crane has the behavioral mechanisms to identify the relationship.The study content about breeding behavioral mechanisms of Red-crowned Crane was more abundant,and puts forward some suggestions for studying deeply and founding reasonable breeding system for Semi-captive Red-crowned Crane population.

关 键 词：丹顶鹤 AFLP 亲缘关系 

分 类 号：Q953[生物学—动物学]