磁刺激对大鼠急性脊髓损伤后神经细胞凋亡、B细胞淋巴瘤/白血病基因-2及caspase-3基因表达的影响  

The effects of magnetic stimulation on nerve cell apoptosis and the expression of B cell lymphoma/leukemia gene 2 and the easpase 3 gene after spinal cord injury

作　　者：刘传珍[1] 熊飞[1] 吕玉华[1] 张其梅[1] 杨俊[1] 李耀彩[1] 张强[1] 周敬华[1] 

机构地区：[1]三峡大学第一临床医学院,宜昌市中心人民医院神经内科,宜昌443003

出　　处：《中华物理医学与康复杂志》2010年第10期735-739,共5页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的 观察磁刺激治疗对急性脊髓损伤（SCI）大鼠神经细胞凋亡、B细胞淋巴瘤/白血病-2基因（Bcl-2）、半胱氨酸蛋白酶-3（caspase-3）基因表达的影响.方法 将60只大鼠随机分成磁刺激组、模型组及假手术组.采用脊髓离断法将磁刺激组、模型组大鼠制成急性SCI模型,假手术组大鼠手术过程中未离断脊髓.磁刺激组大鼠于SCI后第6,12,24及72小时时给予磁刺激治疗,模型组及假手术组大鼠则于相同时间点予假磁刺激.各组分别于上述时间点磁刺激（或假磁刺激）治疗结束后2 h各取5只大鼠处死,取受损脊髓组织制成切片,采用TUNEL法检测神经细胞凋亡情况,选用免疫组化技术检测标本中Bcl-2及caspase-3基因表达.结果 假手术组大鼠受损脊髓中有散在凋亡细胞分布,模型组大鼠可见大量凋亡细胞,磁刺激组大鼠凋亡细胞数量显著少于模型组,组间差异具有统计学意义（P＜0.05）;模型组及磁刺激组在各观察时间点其Bcl-2及caspase-3表达均明显高于假手术组水平（P＜0.05）;磁刺激组大鼠Bcl-2表达显著高于模型组,caspase-3表达显著低于模型组,组间差异均具有统计学意义（P＜0.05）.结论 SCI后早期介入磁刺激治疗,能显著上调受损脊髓部位Bcl-2表达,下调caspase-3表达,从而尽可能抑制神经细胞凋亡,促进受损脊髓功能恢复.Objective To study the effect of magnetic stimulation on the expression of B cell lymphoma/leukemia gene 2 （ Bcl-2 ） and caspase-3 genes, and the apoptosis of neurons in rats with spinal cord injury （SCI）.Methods Sixty rats were randomly divided into a magnetic stimulation group, a model group and a sham-operation group. An SCI model was established in the magnetic stimulation and model groups. The magnetic stimulation was applied at the 6th, 12th, 24th and 72nd hour after the operation to the rats in the magnetic stimulation group, and sham magnetic stimulation was given to the model group and sham-operation group rats at the same time points. Two hours after treatment, 5 rats of each group were sacrificed and their injured spinal cords were sectioned. The gene expressions were detected using immunohistochemical techniques, and apoptosis of neurons was observed by the TUNEL method. Results Few apoptotic cells were found in the sham-operation group, but more were found in the model group. Apoptotic cells in the magnetic stimulation group were significantly fewer than in the model group. The expression of both Bcl-2 and caspase-3 in the magnetic stimulation and model groups was significantly higher than in the sham-operation group at the different time points. Expression of Bcl-2 in the magnetic stimulation group was significantly higher than in the model group, but expression of caspase-3 in the magnetic stimulation group was significantly lower than in the model group. Conclusions Magnetic stimulation up-regulates the expression of Bcl-2 genes and down-regulates the expression of caspase-3 in injured neurons. Magnetic stimulation might have protective and rehabilitative effects after human SCI.

关 键 词：脊髓损伤 磁刺激 凋亡 B细胞淋巴瘤/白血病-2基因 半胱氨酸蛋白酶-3 

分 类 号：R651.2[医药卫生—外科学]