产聚羟基脂肪酸酯细菌的筛选与鉴定  

Isolation and identification of a polyhydroxyalkanoate producing strain

作　　者：李雪云[1] 付时雨[1] 俞霁川[1] 傅恺[1] 陈元彩[1] 张睿哲[1] 刘运思[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广州510640

出　　处：《微生物学报》2010年第11期1488-1493,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30771689);国家高技术研究发展计划(863计划)重点项目(2007AA100704)~~

摘　　要：【目的】聚羟基脂肪酸酯(polyhydroxyalkanoates,PHAs)是一种生物可降解的天然高分子聚酯,本研究的目的是从广东省某啤酒厂废弃的活性污泥中分离筛选PHAs产生菌。【方法】首先,从活性污泥中分离PHAs产生菌。分离方法分3步:(1)富集培养PHAs产生菌;(2)通过苏丹黑B染色法进行初筛;(3)挑选PHAs产量较高的菌株,然后对细胞内提取产物进行分析,最后通过生理生化试验和16SrRNA基因序列分析法对该菌株进行鉴定。【结果】从广东省某啤酒厂的活性污泥样品中筛选获得PHAs产生菌HG-B-1,被鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltophlia)。细胞染色分析、胞内提取物的红外光谱分析表明HG-B-1胞内贮藏物为PHAs。该菌株在以蔗糖为碳源、牛肉膏为氮源的发酵培养基中,37℃振荡培养24h,PHAs产量可达细胞干重的23.4%。【结论】本文从广东省某啤酒厂的活性污泥中筛选得到PHAs产生菌,获得了一株新型的PHAs产生菌,为进一步研究和开发新型的PHAs产生菌提供了菌源和基础资料。[ Objective] We screened and isolated polyhydroxyalkanoate producing bacteria. [ Methods] The strains were isolated from sludge from a beer brewery and screened by Sudan black B staining method. The isolated strains were identified according to their morphological features, physiological and biochemical analysis as well as 16S rRNA gene sequence analysis. The product extracted with hot chloroform from the isolated strain HG-B-1 was confirmed by Fourier transform infrared spectra. [ Results] We isolated a bacterium, HG-B-1, from sludge collected from a beer brewery in Guangdong province, China. The yield of polyhydroxyalkanoates was 23.4% （w/w） based on dried weight of the bacterium cells when HG-B-1 grew in a medium containing saceharose. We analyzed 16S rRNA nucleotide sequence, and ascertained the phylogenetic position of the strain. [ Conclusion ] Strain HG-B-1 with PHAs biosynthesis ability was identified as Stenotrophomonas maltophlia.

关 键 词：聚羟基脂肪酸酯 筛选 鉴定 嗜麦芽寡养单胞菌 

分 类 号：Q93[生物学—微生物学]