纤维蛋白原吸附与构象变化响应在血液相容性中的研究  

Research on Fibrinogen Adsorption and Its Transformation Response in Hemocompatibility

作　　者：金健[1] 万国江[2] 赵安莎[2] 雷跃昌[1] 杨滢[1] 黄楠[2] 吴熹[2] 杨萍[2] 冷永祥[2] 陈俊英[2] 侯明勇[1] 

机构地区：[1]成都铁路中心医院,成都610081 [2]西南交通大学材料先进技术教育部重点实验室,成都610031

出　　处：《生物医学工程学杂志》2010年第5期1062-1065,共4页Journal of Biomedical Engineering

基　　金：国家重点基础研究规划项目资助(G1999064706);国家自然科学基金资助项目(20603027);西南交通大学科研基金项目资助(2007B09)

摘　　要：采用酶联免疫法(ELISA)在钛氧膜(Ti-O)试片及当前心瓣膜材料热解碳(LTIC)为对照的试片上,检测纤维蛋白原(FIG),并分析构象变化的FIG在其表面被吸附的量,同期在6只动物犬股动脉内埋置Ti-O与LTIC试片,6个月后用扫描电镜(SEM)观察表面吸附情况。实验结果显示:FIG均被Ti-O与LTIC吸附,发生构象变化的FIG具有活性并可吸附血小板(PL),但两者的吸附量有显著区别,SEM吸附PL的形态学改变证实了两者的差别。血液内FIG被心血管植入物吸附,其吸附量随心血管植入物结构与成分的不同而不同,FIG与植入物的表面发生电子传递,引起FIG的构象变化,FIG的γ链C端被舒展,出现了FIG结构上的交联点和作用点的暴露,若FIG能保持电稳定,心血管植入物接触后不发生电子传递,才能使心血管植入物的FIG建立良好的血液相容性。In this research,enzyme linked immunoassay（ELISA） was used to assay the fibrinogen（FIG） adsorbed on the Ti-O films and on the low temperature isotropic carbon（LTIC） films which were planted in the femoral arteries of 6 mongrel dogs for six months,respectively.The Ti-O films were planted in the dogs＇ left femoral arteries;the LTIC films as controls were planted in the dogs＇ right femoral arteries.The contents adsorbed in these two kinds of films were examined by scanning electron microscopy（SEM）.The quantities of FIG adhered or denatured on the Ti-O films or LTIC films determined by ELISA,and the platelets adhered on the two kinds of films examined by SEM were of significant difference between the two groups.In the blood vessel,the amount of FIG adhered on biomaterial was related to its component and construction.FIG released electron to the biomaterial and induced the unfolding of C term of the γ-chain of FIG,and the conjugation point and effect point were exposed.In conclusion,the biomaterial,which has the capability for resisting the electron release from FIG as well as for maintaining the invariable electric condition,will have excellent hemocompatibility.

关 键 词：纤维蛋白原 吸附 构象变化 血液相容性 血小板 

分 类 号：R318.08[医药卫生—生物医学工程]