成都地区淋病奈瑟菌耐药谱质粒谱相互关系研究  

Study on Correlation of Antibiotic resistant and Plasmid Profiles of Neisseria Gonorrhoeae Isolated From Patients of Chengdu area.

作　　者：向辉[1,2,3,1] 雍刚 李绍兴[1,2,3,1] 叶梅君 廖巫山[1,2,3,1] 

机构地区：[1]华西医科大学公共卫生学院医检教研室 [2]广州市天河区卫生防疫站 [3]四川省皮肤病性病防治研究所STD检测中心

出　　处：《中国皮肤性病学杂志》1999年第4期222-224,共3页The Chinese Journal of Dermatovenereology

基　　金：四川省科委基金;四川省卫生厅基金

摘　　要：目的为了探讨淋病奈瑟菌耐药谱与质粒谱之间的相互关系。方法我们对本地区１９９１年、１９９６年各５０株标本进行了耐药谱、质粒谱分析。以琼脂扩散法测定其１２种抗生素敏感性；碱裂解加溶菌酶法进行质粒抽提。结果１９９１年分离的标本以同时耐２种抗生素者为主，耐药谱型２４种，质粒谱型５种。１９９６年分离的标本以同时耐３或４种抗生素者为主，耐药谱型３４种，质粒谱型１０种。结论不同时期分离的标本耐药谱、质粒谱变化较大。同一时期分离的标本尽管耐药谱型较分散，但质粒谱型相对稳定。说明本地区淋病奈瑟菌耐药菌株的流行主要是染色体突变所致。Objective To study the correlation of antibiotic resistent and plasmid profiles of Neisseria gonorrhoeae.Methods We analysed the antibiotic resistant profiles and the plasmid profiles of 50 strains in 1991 and 50 strains in 1996.Susceptibilities to 12 antibiotics were determined by agar diffusion method,and plasmids were extracted by alkali with lysozyme.Resulths There were 24 kinds antibiotic resistant profiles and 5 kinds plasmid profiles of 50 strains isolated in 1991.Antibioticresistant profiles increased up to 34 and plasmid profiles increased up to 10 of 50 strains isolated in 1996.Multi resistant strains were mainly to resist 2 antibiotics in 1991,3 or 4 antibiotics in 1996.Conclusions There was a big change in antibiotic resistant profiles and plasmid profiles of strains isolated during different periods,although antibitic resistant had varous profiles,the plasmid profiles kept little change at the same time.It revealed that the epidemic drug resistant were caused by chromatosome mutation.

关 键 词：淋病奈瑟菌 耐药谱 质粒谱 淋病 

分 类 号：R759.2[医药卫生—皮肤病学与性病学] R378.16[医药卫生—临床医学]