膜状成骨与软骨成骨贴附移植后的组织学观察及骨计量学分析  

Histological study and stereologic analysis of membranous versus endochondral onlay bone grafts

作　　者：杨斌[1] 赵敏[1] 熊斌[1] 刘珍君[1] 刘元波[1] 郭永刚 靳晓雷 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院,北京100041

出　　处：《中华整形烧伤外科杂志》1999年第4期280-282,共3页

摘　　要：目的深入探讨骨贴附移植后的变化及不同胚胎来源骨质(膜状成骨和软骨成骨)移植后的差异。方法应用新西兰兔、恒河猴采用荧光素双标记法,在不脱钙骨切片上,选用类骨质宽度、骨质矿化沉积率、荧光标记率等骨动力学指标对骨移植后的再生和改建进行计算机图像分析和定量测量。结果骨体积存留率:膜状成骨为(78.4±3.5)%,软骨成骨为(56±5.1)%;骨矿化沉积率:膜状成骨为(3.60±0.8)μm/d,软骨成骨为(0.92±0.33)μm/d;类骨质宽度;膜状成骨为(14.56±2.69)μm,软骨成骨为(7.38±2.20)μm;荧光标记率:膜状成骨为(91.22±2.69)%,软骨成骨为(51.28±4.11)%。结论膜状成骨移植后较软骨成骨可保持更多的骨质体积,有更为明显的成骨再生能力且可诱导宿主骨成骨的能力不同,膜状成骨优于软骨成骨。Objective The experimental study was to determine the differences in changes in the onlay bone grafts of different embryonic origin.Methods The study was designed to compare the poten- tial for appositional bone growth in membranous and endochondral onlay grafts in New-Zealand rabbits and a rhesus monkey.Results were assessed with volumetric measurement,histological examinations and stere- ologic analysis with double-fluorochrome labeling technique.Results For the membranous and endochon- dral bone grafts,the volumetric retention rate was(78.4±3.5)% and(56.0±5.1)% respectively.The mineralization apposition rate(MAR)was(3.6±0.8)μm/d and(0.92±0.33)μm/d;the osteoid seam width(OSW)was(14.56±2.69)μm and(7.38±2.20)μm ;the tetracycline uptake rate(TUA)was (91.22±2.69)% and(51.28±4.11)%,respectively.Conclusion①It was found that the membra- nous bone graft maintained its volume to a significantly greater extent than the endochondral bone graft; ②Membranous bone grafts exhibited higher survival rate and greater activity than endoehondral bone grafts;②Different changes were also found in the two recipient sites.There was more powerful bone re- generative reaction in the recipient bed of the membranous bone graft than the endochondral one.

关 键 词：帖附骨移植 骨计量学 膜状成骨 软骨成骨 

分 类 号：R687.34[医药卫生—骨科学]