持续高+Gy对豚鼠耳石器的影响  

Effect of sustained high +Gy on otolith organs of guinea pigs

作　　者：戴建国[1,2] 于立身[1,2] 胡美浩 

机构地区：[1]空军航空医学研究所 [2]北京大学生命科学学院

出　　处：《中华航空航天医学杂志》1999年第2期84-86,共3页Chinese Journal of Aerospace Medicine

摘　　要：目的观察持续高Ｇ刺激对耳石器的影响。方法应用原位杂交技术观察了１６只豚鼠在＋３Ｇｙ、＋１０Ｇｙ和＋１８Ｇｙ持续刺激１５ｍｉｎ后，前庭囊斑感觉上皮细胞中ｃ－ｆｏｓｍＲＮＡ和ＢＭＰ１ｍＲＮＡ表达量的变化，并观察了高Ｇ刺激后动物的行为变化。结果三种刺激量均引起豚鼠姿势不稳，出现前庭功能不平衡的症状，并引起中耳腔出血；前庭囊斑感觉上皮中ｃ－ｆｏｓ及ＢＭＰ１ｍＲＮＡ的表达量明显增加。结论＋３Ｇｙ以上的高Ｇ刺激可引起豚鼠出现前庭功能不平衡症状。Objective To investigate the effect of sustained acceleration on otolith organs. Methods Sixteen albino guinea pigs served as subjects. They were divided into 4 groups: 1 control group and 3 experimental groups. The 3 experimental groups underwent 3 Gy,10 Gy and 18 Gy exposure for 15 min respectively, and the postural consequences were observed. The animals were killed 4 h later and the gene expressions of BMP1 and cfos in otolith organs were analyzed by in situ hybridization. ResultsZEleven out of 16 guinea pigs showed postural imbalance after 3 Gy exposure.The instability was mainly a tendency of falling sideward, which is similar to the symptoms of vestibular functional imbalance.Blood clots were found in the middle ear cavity.The higher the +Gy, the severer the symptoms became.BMP1 and cfos gene expressions in the neuroepithelium of macula of experimental groups were significantly higher than those of control group. Conclusions The high G may cause the postural imbalance and change of BMP1 and cfos gene expression in the sensory epithelium of macula in guinea pigs.

关 键 词：加速度 内耳 原位杂交 骨形态发生蛋白 基因表达 

分 类 号：R852.21[医药卫生—航空、航天与航海医学]