经前期综合征肝气逆证猕猴脑组织差减cDNA文库的构建  

Construction of the subtractive cDNA library of premenstrual syndrome macaque with liver-Qi invasion syndrome

作　　者：乔寒子[1] 范立昌 魏盛[3] 张惠云[3] 

机构地区：[1]广州中医药大学附属第二临床医学院,广东广州510120 [2]山东省莒县人民医院,山东莒县276500 [3]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355

出　　处：《山东中医药大学学报》2010年第6期526-529,共4页Journal of Shandong University of Traditional Chinese Medicine

基　　金："973"计划资助课题(编号:2006CB504804);山东省科技攻关项目课题(编号:2006GGB02001)

摘　　要：构建经前期综合征(PMS)肝气逆证猕猴与正常猕猴大脑边缘系统正向与反向差减文库,并对猕猴cDNA文库进行了系统测序和分析,以期筛选出与PMS肝气逆证相关的基因,尤其是与PMS肝气逆证有关的一些神经递质、性激素受体的基因。差减文库构建成功,为弄清PMS肝气逆证差异表达基因奠定了基础。We constructed limbic system subtractive cDNA library of normal macaque and premenstrual syndrome （PMS） macaque with liver-Qi invasion syndrome. The gene sequences in macaque cDNA library were detected and analyzed systematically. Some genes especially neurotransmitter and hormone receptors genes were selected which related to PMS with liver-Qi invasion syndrome. Subtractive cDNA library construction successfully provided solid foundation to clarify differential expression genes of PMS with liver-Qi invasion syndrome.

关 键 词：PMS肝气逆证 猕猴模型 差减CDNA文库 抑制性消减杂交 

分 类 号：R285.5[医药卫生—中药学]