早期CKD大鼠继发性甲旁亢与心脏病变相关关系研究  

The Relationship between Secondary Hyperparathyroidism and Heart Damages in Early Chronic Kidney Disease Rats

作　　者：杜国伟[1] 鲍晓荣[1] 

机构地区：[1]复旦大学附属金山医院肾内科,上海200540

出　　处：《中国中西医结合肾病杂志》2010年第10期869-871,943,共4页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:研究早期慢性肾脏疾病(chronic kidney disease,CKD)大鼠继发性甲状旁腺功能亢进与心脏病变之间的关系。方法:通过单侧肾切除加高磷饲料喂养的方法建立早期CKD大鼠继发性甲旁亢的动物模型,并分析其心脏病变情况及两者之间的关系。结果:(1)实验组大鼠血尿素氮及血清胱抑素C水平均明显高于对照组(P<0.01),但实验组和对照组之间血肌酐、血尿酸水平差异无统计学意义(P>0.05),且病理改变较轻,表明早期CKD模型制作是成功的。(2)实验组与对照组大鼠之间心脏/体重比值差异无统计学意义(P>0.05),但病理提示实验组大鼠心肌细胞肥大,心肌间质纤维化明显,免疫组化测定显示心肌组织内Ⅰ型和Ⅲ型胶原明显增加(P<0.01)。(3)实验组大鼠血磷和PTH水平均明显高于对照组(P<0.01),两组间血钙水平差异无统计学意义(P>0.05),通过单因素相关分析发现大鼠心脏Ⅰ型胶原阳性面积百分比和Ⅲ型胶原阳性面积百分比均与血磷和PTH呈显著正相关(P<0.01)。结论:在通过单侧肾切除加高磷饲料喂养的方法建立的早期CKD动物模型中,发现大鼠心脏结构损害明显,心肌间质纤维组织增生和胶原增多,并且其严重程度与血磷和PTH水平相关。Objective：To study the early chronic kidney disease （chronic kidney disease,CKD） in rats with secondary hyperparathyroidism and heart disease,the relationship between variables.Methods：Unilateral nephrectomy method of increasing the height of phosphorus diet for early CKD secondary hyperparathyroidism in rats in animal models,and analyze the heart attack situation and the relationship between the two.Results：（1） experimental group blood urea nitrogen and serum cystatin C levels were significantly higher （P0.01）,but the experimental group and control group serum creatinine,serum uric acid levels were not significantly different （P0.05）,and the pathological changes of light,that early CKD model making is successful.（2） the experimental group and control group between the heart / body weight ratio was no significant difference （P0.05）,but the experimental group suggest the pathological cardiac hypertrophy,myocardial interstitial fibrosis,immunohistochemistry tests showed that myocardial tissue of type Ⅰ and Ⅲ collagen increased significantly （P0.01）.（3）experimental group phosphorus and PTH were significantly higher （P0.01）,serum calcium levels between the two groups no significant difference （P0.05）,by single factor correlation analysis showed that rat heart type Ⅰ collagen positive area percentage and the percentage of positive area of type Ⅲ collagen were associated with serum phosphorus and PTH were significantly correlated （P0.01）.Conclusion：The unilateral nephrectomy by feeding phosphorus method of increasing the height of the early establishment of animal models of CKD,we found significant structural damage in rat heart,myocardial interstitial fibrosis and collagen increased and their severity and the serum phosphorus and PTH level of correlation.

关 键 词：早期 慢性肾脏疾病 心脏病变 继发性甲旁亢 

分 类 号：R692[医药卫生—泌尿科学]