永生化人骨髓基质干细胞系的生物学特征  

Biological characters of immortalized human bone marrow stromal cell line

作　　者：滕勇[1,2] 胡蕴玉[1] 宿学家[3] 李旭升[1] 白建萍[1] 张德志[1] 

机构地区：[1]第四军医大学西京医院骨科研究所,西安710032 [2]解放军乌鲁木齐总医院骨一科,乌鲁木齐830000 [3]山东泰安解放军88医院心胸外科,271000

出　　处：《中国矫形外科杂志》2010年第20期1719-1722,共4页Orthopedic Journal of China

基　　金：国家863计划资助项目(编号:715-009-0150);兰州军区医药卫生课题(编号:LXH2007014)

摘　　要：[目的]明确永生化人骨髓基质干细胞系MSCxj的生物学特征。[方法]MSCxj细胞在连续体外培养过程中,相差显微镜及透射显微镜观察细胞的形态特征;计算群体倍增时间,测定细胞增殖周期,计算克隆形成率等生长增殖特征并与原代人骨髓基质干细胞BMSCs-2比较。[结果]细胞形态为成纤维细胞样,细胞器发达;群体倍增时间40.8 h,较BMSCs-2的43.82 h短,MSCxj生长增殖活跃;MSCxj克隆形成率10.9%,BMSCs-2为11.5%,具有克隆形成能力。[结论]MSCxj细胞保持了人骨髓基质干细胞的生物学特征。[Objective]To explicate the biological characters of an immortal human bone marrow stromal cell line MSCxj.[Method]The morphology,growth and proliferation of the cells were observed during continuous culture in vitro and compared with that of human bone marrow stromal cells（BMSCs-2）.[Result]The MSCxj cells showed fibroblast-like feature with rich in organelle.The population doubling time（PDT） was 40.8h,it was shorter than the 43.82h of MSCxj＇s PDT.The rate of clone forming was 10.9%,the BMSC-2 was 11.5%.The MSCxj cell grew and proliferated well and were able to form cell clone in vitro.[Conclusion]The MSCxj remains the biological characters of human bone marrow stromal cells.

关 键 词：骨髓基质干细胞 人 生物学 细胞系 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]